Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 5, Problem 2CA
Quality control staff in a sterilization unit of a hospital used a simple stain to determine whether bacteria were present in sterilized materials. A simple stain of sterile saline used for respiratory therapy revealed the presence of bacteria. Is the saline contaminated?
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Chapter 5 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Ch. 5 - Of what value is a simple stain?Ch. 5 - What is the purpose of heat-fixing the smear?Ch. 5 - Another method of fixing smears is to use methanol...Ch. 5 - In heat-fixing, what would happen if too much heat...Ch. 5 - Methylene blue can be prepared as a basic stain or...Ch. 5 - Prob. 2CTCh. 5 - Bacteria can be seen without staining. Why, then,...Ch. 5 - Quality control staff in a sterilization unit of a...
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- A surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. What must be done with the bacterial sample after it is processed in the sterilizer?arrow_forwardWhat is the physical appearance (solid, liquid, semi-solid) of following media: agar platearrow_forwardWhat is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tubearrow_forward
- Which of the following is/are true regarding the acid-fast stain? (There may be more than one correct answer.) Non-acid-fast microbes appear blue in a completed acid-fast stain. O Acid-fast cells retain the primary dye after treatment with acid-alcohol. O It is used to identify members of the genus Mycobacterium. V Acid-fast cells appear bright pink/red in a completed acid-fast stain. O If cells are acid-fast, they are gram-negative.arrow_forwardIn the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?arrow_forwardInfection quality control staff in a sterilization unit of a hospital used a simple stain to determine whether bacteria were present in sterilized materials. A simple stain of sterile saline used for respiratory therapy revealed the presence of bacteria. Is the saline contaminated?arrow_forward
- In preparing a bacterial smear for staining, heat fixation is done after the smear dries up.a.) Give the purpose of heat fixation.b.) What can be observed in wet mounts or hanging-drop slides that cannot be observed in heat-fixed slides?arrow_forwardDisinfectants and Sterilization Procedures Complete the table by putting (+) for growth of microorganisms or (–) absence of growth of microorganisms. Disinfectant Concentration Growth after time of exposure (minutes) Control 5 minutes 10 minutes 15 minutes Sodium hypochlorite 5% Sodium hypochlorite 0.05% Alcohol 70% Alcohol 40% Iodophor (Betadine) 10% solution Mouthwash Cetylpyridinium Chloride - 0.075% Sodium Fluoride - 0.05%arrow_forwardIs is best to observe slides of bacteria stained with the acid fast stain under high dry magnification. True or falsearrow_forward
- Provide three reasons why the use of aseptic technique is essential when handling microbial cultures in the laboratory.arrow_forwardName a few group of microorganisms where Grams staining will not work and/or not appropriate to use. Why?arrow_forwardExplain why only gram-negative cells undergo decolorization during the gram staining procedure. Cite the purpose of each of the following reagents in a differential staining procedure. Primary stain Mordant Decolorizing Agent Counter stain What might happen if the Gram staining procedure is performed on a culture incubated for a little over a dayarrow_forward
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