The effects of inhalation of so much dirty air in which the defense mechanisms of respiratory system cannot function effectively. Introduction: The process of gas exchange that occurs between an organism and its environment is known as respiration. For cellular respiration , most of the animal cells need a continuous oxygen supply. During respiration, oxygen is inhaled from the environment by the animal, and then transported to individual cells of the body. Similarly, carbon dioxide produced by the cell during cellular respiration is exhaled from the body to the environment.

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Definition Definition Set of metabolic processes and reactions that produce energy from organic substances such as glucose. Cellular respiration takes place in the organism’s cells, where the chemical energy from nutrients or molecules of oxygen is converted into adenosine triphosphate (ATP). Cellular respiration that occurs in the presence of oxygen is called aerobic respiration; in the absence of oxygen, it is called anaerobic respiration.

Chapter 46.4, Problem 2C

Summary Introduction

To explain: The effects of inhalation of so much dirty air in which the defense mechanisms of respiratory system cannot function effectively.

Introduction: The process of gas exchange that occurs between an organism and its environment is known as respiration. For cellular respiration, most of the animal cells need a continuous oxygen supply. During respiration, oxygen is inhaled from the environment by the animal, and then transported to individual cells of the body. Similarly, carbon dioxide produced by the cell during cellular respiration is exhaled from the body to the environment.

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a) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following: Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng Final recovery volume: 0.50 mL Volume plated: 0.25 mL Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) b) Using the equation above, calculate the transformation efficiency. c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?

a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates? b) Predict the growth you would expect to see on each of the following plates: – DNA ___________________________________________________________ – DNA/+Amp ______________________________________________________ +DNA/+Amp ______________________________________________________ +DNA/+Amp/+IPTG _________________________________________________

1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids? 2) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following: Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng Final recovery volume: 0.50 mL Volume plated: 0.25 mL Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) 3) Using the equation above, calculate the transformation efficiency. 4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?

Answer 2

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Definition Definition Set of metabolic processes and reactions that produce energy from organic substances such as glucose. Cellular respiration takes place in the organism’s cells, where the chemical energy from nutrients or molecules of oxygen is converted into adenosine triphosphate (ATP). Cellular respiration that occurs in the presence of oxygen is called aerobic respiration; in the absence of oxygen, it is called anaerobic respiration.

Chapter 46.4, Problem 2C

Summary Introduction

To explain: The effects of inhalation of so much dirty air in which the defense mechanisms of respiratory system cannot function effectively.

Introduction: The process of gas exchange that occurs between an organism and its environment is known as respiration. For cellular respiration, most of the animal cells need a continuous oxygen supply. During respiration, oxygen is inhaled from the environment by the animal, and then transported to individual cells of the body. Similarly, carbon dioxide produced by the cell during cellular respiration is exhaled from the body to the environment.

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Answer 3

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Definition Definition Set of metabolic processes and reactions that produce energy from organic substances such as glucose. Cellular respiration takes place in the organism’s cells, where the chemical energy from nutrients or molecules of oxygen is converted into adenosine triphosphate (ATP). Cellular respiration that occurs in the presence of oxygen is called aerobic respiration; in the absence of oxygen, it is called anaerobic respiration.

Chapter 46.4, Problem 2C

Summary Introduction

To explain: The effects of inhalation of so much dirty air in which the defense mechanisms of respiratory system cannot function effectively.

Introduction: The process of gas exchange that occurs between an organism and its environment is known as respiration. For cellular respiration, most of the animal cells need a continuous oxygen supply. During respiration, oxygen is inhaled from the environment by the animal, and then transported to individual cells of the body. Similarly, carbon dioxide produced by the cell during cellular respiration is exhaled from the body to the environment.

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Answer 4

Question

Definition Definition Set of metabolic processes and reactions that produce energy from organic substances such as glucose. Cellular respiration takes place in the organism’s cells, where the chemical energy from nutrients or molecules of oxygen is converted into adenosine triphosphate (ATP). Cellular respiration that occurs in the presence of oxygen is called aerobic respiration; in the absence of oxygen, it is called anaerobic respiration.

Chapter 46.4, Problem 2C

Summary Introduction