Campbell Biology Custom Stony Brook 10 Th Edition
10th Edition
ISBN: 9781269870818
Author: Reece Urry Cain Wasserman Minorsky Jackson
Publisher: PEARSON
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Chapter 46, Problem 4TYU
Summary Introduction
Introduction:
The menstrual cycle in females is a regular monthly process in which the cellular lining of the uterus is made ready for pregnancy. In case pregnancy does not occur, the lining is shed off during menstruation. Hormones secreted from ovaries and pituitary glands control the cycle. Hormones control the release of an egg from an ovary. Estrogen, luteinizing hormone (LH), andfollicle-stimulating hormone (FSH)are the hormones that control the menstrual cycle.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 46 Solutions
Campbell Biology Custom Stony Brook 10 Th Edition
Ch. 46.1 - Compare and contrast the outcomes of asexual and...Ch. 46.1 - Parthenogenesis is the most common form of asexual...Ch. 46.1 - WHAT IF? If a hermaphrodite self-fertilizes, will...Ch. 46.1 - Prob. 4CCCh. 46.2 - How does internal fertilization facilitate life on...Ch. 46.2 - What mechanisms have evolved in animals with (a)...Ch. 46.2 - Prob. 3CCCh. 46.3 - Why might frequent use of a hot tub make it harder...Ch. 46.3 - Prob. 2CCCh. 46.3 - WHAT IF? If each vas deferens in a male was...
Ch. 46.4 - How are the functions of FSH and LH in females and...Ch. 46.4 - How does an estrous cycle differ from a menstrual...Ch. 46.4 - WHAT IF? If a human female begins taking...Ch. 46.4 - Prob. 4CCCh. 46.5 - Prob. 1CCCh. 46.5 - In what ways are tubal ligation and vasectomy...Ch. 46.5 - Prob. 3CCCh. 46 - Would a pair of haploid offspring produced by...Ch. 46 - Identify which of the following, if any, are...Ch. 46 - How does the difference in size and cellular...Ch. 46 - Prob. 46.4CRCh. 46 - Prob. 46.5CRCh. 46 - Prob. 1TYUCh. 46 - In male mammals, excretory and reproductive...Ch. 46 - Prob. 3TYUCh. 46 - Prob. 4TYUCh. 46 - Prob. 5TYUCh. 46 - Prob. 6TYUCh. 46 - Prob. 7TYUCh. 46 - Prob. 8TYUCh. 46 - DRAW IT In human spermatogenesis, mitosis of a...Ch. 46 - Prob. 10TYUCh. 46 - SCIENTIFIC INQUIRY You discover a new egg-laying...Ch. 46 - WRITE ABOUT A THEME: ENERGY AND MATTER In a short...Ch. 46 - SYNTHESIZE YOUR KNOWLEDGE A female Komodo dragon...
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- is often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forwardWhich of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forwardAn unrooted tree containing ten unrelated species can become rooted by adding a descendant group related to two of the species. an unrelated outgroup. O a distantly related outgroup. O a descendant related to only one of the species.arrow_forward
- What is the most appropriate purpose of building a phylogenetic tree? They look awesome You can use a tree to compare morphological characteristics of organisms It can be used to establish and analyze evolutionary relationships between species All of the abovearrow_forwardWhich of the following sequencing techniques can identify down to the strain level? O Multilocus sequence typing Genomic fingerprinting Whole genome sequencing OSNP analysis All of the abovearrow_forwardWhat is the "gold standard" that is currently applied to species designations in microbiology? 97% between species: 50% among whole genome 90% between species: 75% among whole genome 99% between species; 97% among whole genome 97% between species: 70% among whole genome Onone of the abovearrow_forward
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