MICROBIOLOGY: EVOLV.SCI.-W/ACCESS>CI<
4th Edition
ISBN: 9780393622805
Author: SLONCZEWSKI
Publisher: Norton custom
expand_more
expand_more
format_list_bulleted
Concept explainers
Question
Chapter 4.4, Problem 6TQ
Summary Introduction
To review:
The effects on the growth curve, in case the medium have two carbon sources and one of them is a preferred carbon source of growth-limiting concentration and the second is a nonpreferred source.
Introduction:
The artificial media used for culturing the microorganism is referred to as the culture media. The microorganism is generally cultured in a liquid medium with specific constituents that reveal the effects of the changing conditions on the microbial growth. This type of culture is termed as batch culture.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
A culture consisting of 100 litres of nutrient medium containing 12 g l-1 of growth limiting substrate is inoculated with 10g of bacteria in a batch fermenter. The yield of biomass from substrate is 0.05 g g-1 . The maintenance coefficient is 2.0 g g-1 h -1 . The specific rate of product formation due to maintenance is 1.0 h-1 . The maximum specific growth rate of the organism is 0.3 h -1 . YPX= 7.7 and product formation is directly linked with metabolism.
a) What is the time required to produce 15 g of biomass?
!!!!! ANS IS 3.07 HRS!!!!
STEPS on how to reach this please
Colonies have grown in a nutrient agar plate. However, it did not grow along the lines of streaking. Is this normal? If not, what could be the problem?
What is the advantage of the plating method over an electronic cell counting method in counting cells?
Why does turbidity lose reliability at high cell concentrations when the culture reaches the stationary phase?
Chapter 4 Solutions
MICROBIOLOGY: EVOLV.SCI.-W/ACCESS>CI<
Ch. 4.1 - Prob. 1TQCh. 4.2 - Prob. 1TQCh. 4.2 - Prob. 2TQCh. 4.3 - Prob. 1TQCh. 4.3 - Prob. 2TQCh. 4.3 - Prob. 3TQCh. 4.3 - Prob. 4TQCh. 4.4 - Prob. 1TQCh. 4.4 - Prob. 2TQCh. 4.4 - Prob. 3TQ
Ch. 4.4 - Prob. 4TQCh. 4.4 - Prob. 5TQCh. 4.4 - Prob. 6TQCh. 4.4 - Prob. 7TQCh. 4.4 - Prob. 8TQCh. 4.6 - Prob. 1TQCh. 4 - Prob. 1RQCh. 4 - Prob. 2RQCh. 4 - Prob. 3RQCh. 4 - Prob. 4RQCh. 4 - Prob. 5RQCh. 4 - Prob. 6RQCh. 4 - Prob. 7RQCh. 4 - Prob. 8RQCh. 4 - Prob. 9RQCh. 4 - Prob. 1TQCh. 4 - Prob. 2TQCh. 4 - Prob. 3TQCh. 4 - Prob. 4TQCh. 4 - Prob. 5TQCh. 4 - Prob. 6TQCh. 4 - Prob. 7TQ
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Why is the colony diameter of the yeast culture grown on potato dextrose agar is larger than those grown on acetate agar? Give and discuss the reasons for this different in growth.arrow_forward(1) What is the difference between specific growth rate and generation time? (2) Why is there a need to adjust the optical density of a pre-culture against a McFarland standard or measure it using a spectrophotometer?arrow_forwardWhy do we use turbidity as a method to measure bacterial growth? How does it work? How do you interpret the theoretical and actual results of turbidity measurements? Can you think of advantages and disadvantages to using this type of method?arrow_forward
- 1(a) 1. What is the theory upon which intermittent sterilization is based? (B) Is dry or moist heat more efficient as a sterilizing agent? Why? (C) When would it be necessary to use aseptic techniques when inoculating a tube of nutrient broth with dirt? (D)Why do some microorganisms characteristically form a growth of the pellicle type? What environmental factors might alter the formation of a pellicle? (This is not a graded assignment)arrow_forwardIf you have a 15 mg/100 ml stock solution of GA3 and you need a 1 mg GA3 in 25 ml, how much stock solution would you add to 125 ml of medium? how to calculate these kind of question in tissue culture media preperationarrow_forwardA soil sample is placed in liquid and the number of bacteria in the sample determined in two ways: (1) colony count and (2) direct microscopic count. How would the results compare?a) Methods 1 and 2 would give approximately the same results.b) Many more bacteria would be estimated by method 1.c) Many more bacteria would be estimated by method 2.d) Depending on the soil sample, sometimes method 1 would be higher and sometimes method 2 would be higher.arrow_forward
- What is the best growth medium that contains glucose that can be used to acclimatize bacteria? and why?arrow_forwardDraw a semi-logarithmic diagram showing the killing kinetics of a vegetative bacterial culture, assuming appropriate temperature, e.g. 100°C. Label the axes. 1.) What are the kinetics for killing bacterial spores at 100°C and 121°C 2.) and what about a mixture of different heat-resistant vegetative cells?arrow_forwardWhich B. megaterium culture, a culture in the exponential growth phase or a culture in the stationary phase, would show the best survival following exposure to UV radiation? Explain.arrow_forward
- A study was conducted on the impact of phytohormones on medicinal stem explants.The stem explants were cultured onto MS media containingdifferent types of phytohormones as listed in Table below. Predict the resultsobtained after 4 weeks with justifications and conclude the results youpredictedarrow_forwardB) A total of 2.5 x 105 cells were harvested from the patient. You need the cells to fill a scaffold with dimensions of 20mm x 20mm x 4mm at a final density of 2 x 104 cells/mm³. After culturing the harvested cells, you found that they have a doubling time of 36 hours. Given the growth rate is proportional to the number of cells, determine the time it will take to achieve the desired cell count. Show your work.arrow_forwardExplain the expressions of primary culture, cell haH, and continuous cell haH. Explain the reason why some cells grow in suspension in culture and some grow by adhering. Give examples of these two cell types.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
cell culture and growth media for Microbiology; Author: Scientist Cindy;https://www.youtube.com/watch?v=EjnQ3peWRek;License: Standard YouTube License, CC-BY