Foundations in Microbiology
9th Edition
ISBN: 9780073522609
Author: Kathleen Park Talaro, Barry Chess Instructor
Publisher: McGraw-Hill Education
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Chapter 4.4, Problem 20CYP
Summary Introduction
Introduction:
Bacterial chromosomes long circular single stranded DNA that are highly coiled and aggregate at the centre of cell, known as nucleoid. They carry genes required for growth and reproduction. Plasmids are small circular single stranded DNA that confer various functions to the cell but are not necessary for growth and reproduction.
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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
Chapter 4 Solutions
Foundations in Microbiology
Ch. 4.1 - Describe the fundamental characteristics of cells.Ch. 4.1 - Identify the primary properties that define life...Ch. 4.1 - Prob. 1CYPCh. 4.1 - Name several general characteristics that could be...Ch. 4.2 - Describe the generalized anatomy of bacterial...Ch. 4.2 - Prob. 4ELOCh. 4.2 - Describe the structure and position of bacterial...Ch. 4.2 - Explain how flagella influence motility and motile...Ch. 4.2 - Prob. 7ELOCh. 4.2 - Define glycocalyx, and describe its different...
Ch. 4.2 - What other microbial groups besides bacteria have...Ch. 4.2 - Prob. 4CYPCh. 4.2 - How does the flagellum dictate the behavior of a...Ch. 4.2 - Differentiate between the structure and functions...Ch. 4.2 - Prob. 7CYPCh. 4.2 - Prob. 8CYPCh. 4.2 - Prob. 9CYPCh. 4.2 - Explain how the bacterial glycocalyx and certain...Ch. 4.3 - Prob. 9ELOCh. 4.3 - Outline the structure and functions of cell walls,...Ch. 4.3 - Contrast the major structure of gram-positive and...Ch. 4.3 - Prob. 12ELOCh. 4.3 - Relate the characteristics of other types of cell...Ch. 4.3 - Prob. 14ELOCh. 4.3 - Prob. 11CYPCh. 4.3 - Prob. 12CYPCh. 4.3 - Prob. 13CYPCh. 4.3 - How does the precise structure of the cell walls...Ch. 4.3 - Prob. 15CYPCh. 4.3 - Prob. 16CYPCh. 4.3 - Describe the medical impact of the cell walls of...Ch. 4.3 - Prob. 18CYPCh. 4.3 - Prob. 19CYPCh. 4.4 - Prob. 15ELOCh. 4.4 - Prob. 16ELOCh. 4.4 - Characterize the bacterial ribosomes and...Ch. 4.4 - Prob. 18ELOCh. 4.4 - Prob. 19ELOCh. 4.4 - Discuss the resistance and significance of...Ch. 4.4 - Prob. 20CYPCh. 4.4 - What is unique about the structure of bacterial...Ch. 4.4 - Prob. 22CYPCh. 4.4 - Prob. 23CYPCh. 4.4 - Describe the way endospores are formed, their...Ch. 4.4 - Prob. 25CYPCh. 4.4 - Why are spores so difficult to destroy?Ch. 4.5 - Describe the shapes of bacteria and their possible...Ch. 4.5 - Prob. 22ELOCh. 4.5 - Outline the size ranges among bacteria and in...Ch. 4.5 - Prob. 27CYPCh. 4.5 - Prob. 28CYPCh. 4.5 - What are vibrios and coccobacilli?Ch. 4.5 - Prob. 30CYPCh. 4.5 - Prob. 31CYPCh. 4.5 - Prob. 32CYPCh. 4.6 - Prob. 24ELOCh. 4.6 - Prob. 25ELOCh. 4.6 - Outline a basic system of bacterial taxonomy.Ch. 4.6 - Explain the species and subspecies levels for...Ch. 4.6 - Prob. 33CYPCh. 4.6 - Prob. 34CYPCh. 4.6 - Prob. 35CYPCh. 4.7 - Differentiate various groups of photosynthetic...Ch. 4.7 - Characterize the types of obligate intracellular...Ch. 4.7 - Prob. 30ELOCh. 4.7 - Prob. 31ELOCh. 4.7 - Prob. 32ELOCh. 4.7 - Prob. 36CYPCh. 4.7 - Name two main groups of obligate intracellular...Ch. 4.7 - Explain the characteristics of archaea that...Ch. 4.7 - Prob. 39CYPCh. 4.7 - Describe the three major archaeal lifestyles and...Ch. 4.L1 - Prob. 1MCQCh. 4.L1 - Viruses are not considered living things because...Ch. 4.L1 - Prob. 3MCQCh. 4.L1 - Prob. 4MCQCh. 4.L1 - Pili are appendages in _________ bacteria that...Ch. 4.L1 - Prob. 6MCQCh. 4.L1 - Which of the following is a primary bacterial cell...Ch. 4.L1 - Which of the following is present in both...Ch. 4.L1 - Metachromatic granules are concentrated ________...Ch. 4.L1 - Prob. 10MCQCh. 4.L1 - Prob. 11MCQCh. 4.L1 - Prob. 12MCQCh. 4.L1 - Prob. 13MCQCh. 4.L1 - Prob. 14MCQCh. 4.L1 - Prob. 15MCQCh. 4.L1 - To which taxonomic group do cyanobacteria belong?...Ch. 4.L1 - Prob. 17MCQCh. 4.L1 - The first living cells on earth would most likely...Ch. 4.L1 - Prob. 1CSRCh. 4.L1 - Prob. 2CSRCh. 4.L1 - What is a biofilm, and how did it form on the...Ch. 4.L1 - Label the parts on the bacterial cell featured...Ch. 4.L1 - Discuss the collection of properties that are used...Ch. 4.L1 - Describe the basic process of biofilm formation.Ch. 4.L1 - Prob. 4WCCh. 4.L1 - Prob. 5WCCh. 4.L2 - Using clay, demonstrate how cocci can divide in...Ch. 4.L2 - Prob. 2CTCh. 4.L2 - Prob. 3CTCh. 4.L2 - Prob. 4CTCh. 4.L2 - a. Name a bacterial group that uses chlorophyll to...Ch. 4.L2 - Propose a hypothesis to explain how bacteria and...Ch. 4.L2 - Prob. 7CTCh. 4.L2 - Prob. 8CTCh. 4.L2 - Describe the shapes and arrangements of bacteria...Ch. 4.L2 - Prob. 1VCCh. 4.L2 - What kinds of cells are shown here? Explain what...
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