LIFE: THE SCIENCE OF BIOLOGY
LIFE: THE SCIENCE OF BIOLOGY
11th Edition
ISBN: 9781319145125
Author: Sadava
Publisher: MAC HIGHER
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Chapter 42.5, Problem 2R
Summary Introduction

To review:

The difference between the mechanism of action of the contragestational pill, such as RU-486 and contraceptive pill, despite acting through progesterone signaling.

Introduction:

Oral contraceptive pills and contragestational pills are taken by women to avoid the unwanted pregnancy. Although both of them involve progesterone signaling, the mechanism of action is different in both the cases.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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