Biology
Biology
10th Edition
ISBN: 9780321794260
Author: Audesirk, Teresa/ Audesirk
Publisher: Pearson College Div
Question
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Chapter 40.1, Problem 1CYL
Summary Introduction

To describe:

The structure of vertebrate skeletal muscle from a whole muscle through individual muscle cells and subcellular structures down to the proteins involved in muscle contraction.

Introduction:

During muscle contraction, the myosin head flexes, pulling the thin filament the thick filament and shortening the sarcomere. The Nervous system controls the contraction of skeletal muscles.

Summary Introduction

To explain:

The sliding filament mechanism of muscle contraction.

Introduction:

The thin and thick filaments include the molecular architecture which allows both to grip and to slide past one another. This provides the contraction of muscles and shortening of the sarcomere and is known as sliding filament mechanism

Summary Introduction

To explain:

The way in which the nervous system causes the contraction of skeletal muscles and the way it determines the strength of contraction.

Introduction:

A skeletal muscle's reserves of ATP are used up after only a few seconds of high-intensity exercise. The muscles used the energy in the stretched form. The Nervous system controls the contraction of skeletal muscles.

Summary Introduction

To distinguish:

Between the fast twitch and slow twitch muscles.

Introduction:

Two different and specified activities are involved in the two types of muscle fibers: fast twitch and slow twitch. They are different forms of myosin which causes to contract slowly and more rapidly.

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Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
a) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following:  Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng  Final recovery volume: 0.50 mL  Volume plated: 0.25 mL  Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) b) Using the equation above, calculate the transformation efficiency. c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?   b) Predict the growth you would expect to see on each of the following plates:         – DNA ___________________________________________________________ – DNA/+Amp ______________________________________________________ +DNA/+Amp ______________________________________________________ +DNA/+Amp/+IPTG _________________________________________________
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