Biology
Biology
10th Edition
ISBN: 9780321794260
Author: Audesirk, Teresa/ Audesirk
Publisher: Pearson College Div
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Chapter 40, Problem 2AC
Summary Introduction

To determine:

The mechanism by which neostigmine restore muscle activity

Introduction:

Myasthenia gravis is a neuromuscular disorder which leads to cause weakness of skeletal muscle. It commonly affects the muscles of eyes, face, and so on. This disorder occurs in the body when signals transmitted from nerve to muscle fiber are disrupted.

Summary Introduction

To determine:

The possibilities that neostigmine could restore the normal muscle function for the entire life.

Introduction:

Myasthenia gravis is a neuromuscular disorder which causes weakness of skeletal muscle. It commonly affects the muscles of eyes, face, and so on. This disorder occurs in the body when signals transmitted from nerve to muscle fiber are disrupted.

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Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
a) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following:  Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng  Final recovery volume: 0.50 mL  Volume plated: 0.25 mL  Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) b) Using the equation above, calculate the transformation efficiency. c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?   b) Predict the growth you would expect to see on each of the following plates:         – DNA ___________________________________________________________ – DNA/+Amp ______________________________________________________ +DNA/+Amp ______________________________________________________ +DNA/+Amp/+IPTG _________________________________________________
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