Biology 2e
2nd Edition
ISBN: 9781947172517
Author: Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher: OpenStax
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Textbook Question
Chapter 40, Problem 22CTQ
What happens in capillaries?
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 40 Solutions
Biology 2e
Ch. 40 - Figure 40.10 Which of the following statements...Ch. 40 - Figure 40.11 Which of the following statements...Ch. 40 - Figure 40.17 Varicose veins are veins that become...Ch. 40 - Why are open circulatory systems advantageous to...Ch. 40 - Some animals use diffusion instead of a...Ch. 40 - Blood flow that is directed through the lungs and...Ch. 40 - White blood cells: can be classified as...Ch. 40 - Platelet plug formation occurs at which point?...Ch. 40 - In humans, the plasma comprises what percentage of...Ch. 40 - The red blood cells of birds differ from mammalian...
Ch. 40 - The heart's internal pacemaker beats by: an...Ch. 40 - During the systolic phase of the cardiac cycle,...Ch. 40 - Card io myocytes are similar to skeletal muscle...Ch. 40 - How do arteries differ from veins? Arteries have...Ch. 40 - High blood pressure would be a result of...Ch. 40 - Describe a closed circulatory systemCh. 40 - Describe systemic circulationCh. 40 - IS. Describe the cause of different blood type...Ch. 40 - List some of the functions of blood in the body.Ch. 40 - How does the lymphatic system work with blood...Ch. 40 - Describe the cardiac cycleCh. 40 - What happens in capillaries?Ch. 40 - How does blood pressure change during heavy...
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