CAMPBELL BIOLOGY,VOL.II >CUSTOM<
17th Edition
ISBN: 9781323803677
Author: Urry
Publisher: PEARSON
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Textbook Question
Chapter 40, Problem 10TYU
SCIENTIFIC INQUIRY Eastern tent caterpillars (Malacosoma americanum) live in large groups in silk nests resembling tents, which they build in trees. They are among the first insects to be active in early spring, when daily temperature fluctuates from freezing to very hot. Over the course of a day, they display striking differences in behavior: Early in the morning, they rest in a tightly packed group on the tent's east-facing surface. In midafternoon, they are on its undersurface, each caterpillar hanging by a few of its legs. Propose a hypothesis to explain this behavior. How could you test it?
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 40 Solutions
CAMPBELL BIOLOGY,VOL.II >CUSTOM<
Ch. 40.1 - What properties do all types of epithelia share?Ch. 40.1 - VISUAL SKILLS Consider the idealized animal in...Ch. 40.1 - WHAT IF? Suppose you are standing at the edge of...Ch. 40.2 - MAKE CONNECTIONS How does negative feedback in...Ch. 40.2 - If you were deciding where to put the thermostat...Ch. 40.2 - Prob. 3CCCh. 40.3 - Prob. 1CCCh. 40.3 - Flowers differ in how much sunlight they absorb....Ch. 40.3 - Prob. 3CCCh. 40.4 - If a mouse and a small lizard of the same mass...
Ch. 40.4 - Prob. 2CCCh. 40.4 - Prob. 3CCCh. 40 - Prob. 40.1CRCh. 40 - Is it accurate to define homeostasis as a constant...Ch. 40 - Given that humans thermoregulate, explain why your...Ch. 40 - Why do small animals breathe more rapidly than...Ch. 40 - Level 1: Knowledge/Comprehension 1. The body...Ch. 40 - Prob. 2TYUCh. 40 - Consider the energy budgets for a human, an...Ch. 40 - Prob. 4TYUCh. 40 - Prob. 5TYUCh. 40 - Prob. 6TYUCh. 40 - Prob. 7TYUCh. 40 - Prob. 8TYUCh. 40 - EVOLUTION CONNECTION In 1847, the German biologist...Ch. 40 - SCIENTIFIC INQUIRY Eastern tent caterpillars...Ch. 40 - SCIENCE. TECHNOLOGY. AND SOCIETY Medical...Ch. 40 - WRITE ABOUT A THEME: ENERGY AND MATTER In a short...Ch. 40 - 13. SYNTHESIZE YOUR KNOWLEDGE These macaques...
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