BIOLOGY-TEXT
BIOLOGY-TEXT
5th Edition
ISBN: 9781260169621
Author: BROOKER
Publisher: MCG
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Chapter 4, Problem 6TY
Summary Introduction

A large network of membranes enclosing the nucleus is known as the endomembrane system. The membranes of the endomembrane system are used to divide cells into organelles. The endomembrane system contains various organelles. Each organelle of the endomembrane system performs a specific role. Golgi apparatus and the endoplasmic reticulum are a part of the endomembrane system and are involved in vesicle transport.

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If cells are treated with colchicine, a drug that promotes microtubule disassembly, the location of the ER and Golgi both change dramatically.  Rather than being spread out quite widely through the cytoplasm, the ER appears to collapse into the area near the nucleus. (Recall that the ER membrane and the outer nuclear envelope are continuous with one another). In contrast, the Golgi stacks disperse from their usually more compact, central location to areas all over the cell. However, if colchicine is removed, and microtubules are allowed to reassemble, both organelles eventually return to their normal distribution in the cells. How do you account for the change in distribution of the ER & Golgi when microtubules are depolymerized? What protein(s), other than tubulins, allow normal cellular organization to be restored once colchicine is removed? (answer should include information to explain both the ER & Golgi)
Various types of vesicle coats have been implicated in membrane traffic pathways such as endocytosis, export from the ER to the Golgi, and transport between Golgi cisternae. But so far, no coat has been identified for the secretory vesicles that carry material from the Golgi to the plasma membrane. Do you think such a coat is likely to exist?
Researchers tried to explain how vesicular transport occurs in cells by attempting to assemble the transport components. They set up microtubular tracks along which vesicles could be transported, and they added vesicles and motor protein kinesin. Yet, when they put everything together, there was no movement or transport of vesicles. What were they missing? endoplasmic reticulum contractile microfilaments OATP an axon intermediate filaments
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