EBK HUMAN ANATOMY & PHYSIOLOGY
1st Edition
ISBN: 9780100659834
Author: AMERMAN
Publisher: YUZU
expand_more
expand_more
format_list_bulleted
Concept explainers
Question
Chapter 4, Problem 4CYR
Summary Introduction
To review:
The given blank spaces in the statement, “The tight junctions make the spaces between cells ________, whereas, desmosomes increase the _________ of a tissue”.
Introduction:
In the extracellular matrix, cells are anchored to proteins. The cells in a tissue are also linked to each other. There are two different ways through which cells are linked, namely, by integral proteins and by cell junctions. There are three main types of cell junctions, namely, tight junction, desmosomes, and gap junctions.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 4 Solutions
EBK HUMAN ANATOMY & PHYSIOLOGY
Ch. 4.1 - What are the four types of tissues, and what are...Ch. 4.1 - What are the two major components of the ECM, and...Ch. 4.1 - What are the three types of protein fibers?...Ch. 4.1 - Prob. 4QCCh. 4.2 - Why is living epithelial tissue limited to a...Ch. 4.2 - Where is the ECM of epithelial tissue located, and...Ch. 4.2 - What are the three basic shapes of epithelial...Ch. 4.2 - What are the differences between simple,...Ch. 4.2 - Prob. 5QCCh. 4.2 - How do exocrine and endocrine glands differ?
Ch. 4.2 - Compare and contrast merocrine and holocrine...Ch. 4.3 - 1. What are the types of cells of connective...Ch. 4.3 - What is the primary component in: a. loose...Ch. 4.3 - What are the primary differences between brown and...Ch. 4.3 - How do the three types of cartilage differ?Ch. 4.3 - How do bone and blood differ from other connective...Ch. 4.4 - What are the two forms of muscle cells, and how do...Ch. 4.4 - Compare and contrast the three types of muscle...Ch. 4.5 - What are the two main cell types in nervous...Ch. 4.5 - 2. What are the three parts of a neuron, and what...Ch. 4.7 - Where are serous and synovial membranes located,...Ch. 4.7 - 2. Where are mucous membranes located, and what...Ch. 4.7 - 3. What is the cutaneous membrane? What is its...Ch. 4.8 - How do regeneration and fibrosis differ?Ch. 4.8 - Which tissues generally heal by regeneration?...Ch. 4.8 - Which factors influence the ability of a tissue to...Ch. 4 - 1 Which of the following best describes the...Ch. 4 - 2. State whether each of the following describes...Ch. 4 - Describe the roles of each of the following...Ch. 4 - Prob. 4CYRCh. 4 - Mark the following statements as true or false. If...Ch. 4 - 6. Match each type of epithelium with its correct...Ch. 4 - Compare and contrast the following pairs of terms:...Ch. 4 - Explain how connective tissues differ from...Ch. 4 - 9. Mark the following statements as true or...Ch. 4 - 10. Match the following types of connective...Ch. 4 - Which of the following statements about muscle...Ch. 4 - 12. Fill in the blanks: _____are the cells of...Ch. 4 - Prob. 13CYRCh. 4 - Which tissues undergo regeneration? Which tissues...Ch. 4 - 1. If you were to cut through epithelial tissue...Ch. 4 - Prob. 2CYUCh. 4 - Prob. 3CYUCh. 4 - The disease pemphigus vulgaris involves a patients...Ch. 4 - Prob. 2AYKACh. 4 - 3. In the disease pulmonary fibrosis, elastic...Ch. 4 - Imagine that a disease turns the simple epithelia...Ch. 4 - Epithelial cells of the kidneys have pumps that...Ch. 4 - Explain why it would be difficult for a mature...Ch. 4 - 7. Predict which organelles are likely to be...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Based on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forwardIn oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the reaction, OTMPD is electron acceptor O cytochrome c is the electron source oxygen is terminal electron acceptor OH2 produced is electron donorarrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have shipped your samples off for sequencing and are now waiting for the results. Out of curiosity (and maybe boredom...) you decide to test your culture for the Catalase and Oxidase enzymes. Upon testing your sample for catalase, you don't see any bubbles; however, you do see a color change to purple during the Oxidase test. What results can you conclude from this? O Catalase-/ Oxidase + O Catalase +/ Oxidase + Catalase + / Oxidase- O Catalase / Oxidase - O None of the abovearrow_forward
- Which of the following is not a strength of using 16S rRNA for phylogenetic analyses? OA. It's cheap OB. It's easy to do C. It can be used to identify all the way down to the strain level OD. Both A & B OE. None of the abovearrow_forwardWhy are molecular approaches important to the field of microbial taxonomy and phylogeny? Phylogenetic inferences based on molecular approaches provide the most robust analysis of microbial evolution currently available. It allows for the collection of a large and accurate dataset from many organisms Almost no fossil record was left by microbes when compared to plants and animals All of the above None of the abovearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forward
- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forwardWhich of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forward
- An unrooted tree containing ten unrelated species can become rooted by adding a descendant group related to two of the species. an unrelated outgroup. O a distantly related outgroup. O a descendant related to only one of the species.arrow_forwardWhat is the most appropriate purpose of building a phylogenetic tree? They look awesome You can use a tree to compare morphological characteristics of organisms It can be used to establish and analyze evolutionary relationships between species All of the abovearrow_forwardWhich of the following sequencing techniques can identify down to the strain level? O Multilocus sequence typing Genomic fingerprinting Whole genome sequencing OSNP analysis All of the abovearrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Human Biology (MindTap Course List)BiologyISBN:9781305112100Author:Cecie Starr, Beverly McMillanPublisher:Cengage LearningBiology: The Unity and Diversity of Life (MindTap...BiologyISBN:9781305073951Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa StarrPublisher:Cengage LearningAnatomy & PhysiologyBiologyISBN:9781938168130Author:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark WomblePublisher:OpenStax College
Human Biology (MindTap Course List)
Biology
ISBN:9781305112100
Author:Cecie Starr, Beverly McMillan
Publisher:Cengage Learning
Biology: The Unity and Diversity of Life (MindTap...
Biology
ISBN:9781305073951
Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher:Cengage Learning
Anatomy & Physiology
Biology
ISBN:9781938168130
Author:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble
Publisher:OpenStax College