BIOCHEMISTRY (LOOSELEAF)-W/ACCESS
BIOCHEMISTRY (LOOSELEAF)-W/ACCESS
9th Edition
ISBN: 9781319425784
Author: BERG
Publisher: Macmillan Higher Education
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Chapter 4, Problem 19P
Interpretation Introduction

(a)

Interpretation:

The type of the radioactive material needs to be determined that is added to the culture medium considering that DNA is supposed to be radioactively labeled but not RNA, in dividing and growing bacterial cell.

Concept introduction:

Nucleotides are organic molecules which act as monomer cells for the formation of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) nucleic acid structures, both of which are vital biomolecules in all life forms on Earth.

Nucleotides are the construction buildings of nucleic acids; they consist of three sub-unit molecules: a core of nitrogen (also recognized as nucleobase), five-carbon acid (ribose or deoxyribose) and at least one set of phosphates.

Interpretation Introduction

(b)

Interpretation:

A product comprising DNA polymerase and primed model DNA must be supplemented with precursors. Also, where core phosphorous atoms are evenly marked with 32P, the location of radioactive atoms in these precursors when the DNA is to be ready should be stated.

Concept introduction:

DNA elongation performs in 5’-3’direction. Chain elongation reaction takes place when 3-hydroxyl group attacks the innermost phosphorus atom of deoxy nucleoside triphosphate. In the DNA replication method where the process is associated with the nuclear matrix, precursors are injected. DNA polymerases catalyze the gradual introduction to a DNA sequence of deoxyribonucleotide components.

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Background Freezing isn't the only challenge in cryopreservation - thawing can be just as difficult. A microwave oven seems like a nice solution, since it deposits energy quickly and microwaves are non-ionizing radiation (they do not cause DNA mutation). However, water absorbs microwaves more effectively than ice does, meaning that the portion of an organ that has already melted will get warmer at a higher rate than the remaining ice – the opposite of what we want! - The transmission of radiation through a weakly absorbing material such as ice or water can be modeled by Beer's law, which assumes that the rate of absorption at a depth x is proportional to the local radiation intensity I(x) times an absorption coefficient, which is often written as μ or a or just µ). Noting that absorption decreases the intensity, we can write a differential equation a Solving the differential equation with the boundary condition on the surface being gives the relationship For a standard microwave oven…
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