BIOCHEMISTRY (LOOSELEAF)-W/ACCESS
BIOCHEMISTRY (LOOSELEAF)-W/ACCESS
9th Edition
ISBN: 9781319425784
Author: BERG
Publisher: Macmillan Higher Education
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Chapter 4, Problem 22P
Interpretation Introduction

Interpretation:

The primer that can be used when a reverse transcriptase activity is supposed to be assayed where polyriboadenylate is in the template needs to be determined. Also, the radioactive nucleotide that is to be used to follow chain elongation needs to be determined.

Concept introduction:

Primer is a single strand of nucleic acid. It is utilized by all the living organisms for the synthesis of DNA. The primers are removed before completion of DNA replication and DNA polymerases fill the gaps in the sequence with DNA. Scientists in the laboratory can design and synthesize DNA primers with particular sequences in a single-stranded DNA molecule that bind to DNA.

In order to perform the polymerase chain reaction, these DNA primres are commonly used to copy pieces. Shellac primers based on oil, latex and pigmented are three different types of primers

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Background Freezing isn't the only challenge in cryopreservation - thawing can be just as difficult. A microwave oven seems like a nice solution, since it deposits energy quickly and microwaves are non-ionizing radiation (they do not cause DNA mutation). However, water absorbs microwaves more effectively than ice does, meaning that the portion of an organ that has already melted will get warmer at a higher rate than the remaining ice – the opposite of what we want! - The transmission of radiation through a weakly absorbing material such as ice or water can be modeled by Beer's law, which assumes that the rate of absorption at a depth x is proportional to the local radiation intensity I(x) times an absorption coefficient, which is often written as μ or a or just µ). Noting that absorption decreases the intensity, we can write a differential equation a Solving the differential equation with the boundary condition on the surface being gives the relationship For a standard microwave oven…
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