Biochemistry, The Molecular Basis of Life, 6th Edition
6th Edition
ISBN: 9780190259204
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 4, Problem 15RQ
Summary Introduction
Introduction:
Standard conditions are the conditions of temperature and pressure that have been standardized and are generally used in laboratory conditions. The standard temperature value is 273.15 Kelvin (K) and 1 atmosphere (atm) is the standard pressure.
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Examine the metabolic pathway. The enzymes that catalyze each step are identified as "e" with a numeric subscript.
e₁
e3
e4
A
B
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1°
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02
e5
e6
e7
E
F
Which enzymes catalyze irreversible reactions?
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es
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e7
Which of the enzymes is likely to be the allosteric
enzyme that controls the synthesis of G?
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e4
es
26
5
e7
An allosteric enzyme that follows the concerted model has an allosteric coefficient (T/R) of 300 in the absence of substrate.
Suppose that a mutation reversed the ratio.
Select the effects this mutation will have on the relationship between the rate of the reaction (V) and substrate
concentration, [S].
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The enzyme would likely follow Michaelis-Menten kinetics.
The plot of V versus [S] would be sigmoidal.
The enzyme would mostly be in the T form.
The plot of V versus [S] would be hyperbolic.
The enzyme would be more active.
Penicillin is hydrolyzed and thereby rendered inactive by penicillinase (also
known as ẞ-lactamase), an enzyme present in some penicillin-resistant
bacteria. The mass of this enzyme in Staphylococcus aureus is 29.6 kDa.
The amount of penicillin hydrolyzed in 1 minute in a 10.0 mL. solution
containing 1.00 x 10 g of purified penicillinase was measured as a
function of the concentration of penicillin. Assume that the concentration of
penicillin does not change appreciably during the assay.
Plots of V versus [S] and 1/V versus 1/[S] for these data are shown.
Vo (* 10 M minute"¹)
7.0
6.0
5.0
4.0
3.0
20
1.0
0.0
о
10
20
30
1/Vo (* 10 M1 minute)
20
103
90
BO
70
50
[S] (* 100 M)
40
50
60
y=762x+1.46 × 10"
[Penicillin] (M)
Amount hydrolyzed (uM)
1
0.11
3
0.25
5
0.34
10
0.45
30
0.58
50
0.61
Chapter 4 Solutions
Biochemistry, The Molecular Basis of Life, 6th Edition
Ch. 4 - Prob. 1QCh. 4 - Prob. 2QCh. 4 - Prob. 1RQCh. 4 - Prob. 2RQCh. 4 - Prob. 3RQCh. 4 - Prob. 4RQCh. 4 - Prob. 5RQCh. 4 - Prob. 6RQCh. 4 - Prob. 7RQCh. 4 - Prob. 8RQ
Ch. 4 - Prob. 9RQCh. 4 - Prob. 10RQCh. 4 - Prob. 11RQCh. 4 - Prob. 12RQCh. 4 - Prob. 13RQCh. 4 - Prob. 14RQCh. 4 - Prob. 15RQCh. 4 - Prob. 16RQCh. 4 - Prob. 17RQCh. 4 - Prob. 18RQCh. 4 - Prob. 19RQCh. 4 - Prob. 20RQCh. 4 - Prob. 21RQCh. 4 - Prob. 22RQCh. 4 - Prob. 23RQCh. 4 - Prob. 24RQCh. 4 - Prob. 25RQCh. 4 - Prob. 26RQCh. 4 - Prob. 27RQCh. 4 - Prob. 28RQCh. 4 - Prob. 29RQCh. 4 - Prob. 30FBCh. 4 - Prob. 31FBCh. 4 - Prob. 32FBCh. 4 - Prob. 33FBCh. 4 - Prob. 34FBCh. 4 - Prob. 35FBCh. 4 - Prob. 36FBCh. 4 - Prob. 37FBCh. 4 - Prob. 38FBCh. 4 - Prob. 39FBCh. 4 - Prob. 40SACh. 4 - Prob. 41SACh. 4 - Prob. 42SACh. 4 - Prob. 43SACh. 4 - Prob. 44SACh. 4 - Prob. 45TQCh. 4 - Prob. 46TQCh. 4 - Prob. 47TQCh. 4 - Prob. 48TQCh. 4 - Prob. 49TQCh. 4 - Prob. 50TQCh. 4 - Prob. 51TQCh. 4 - Prob. 52TQCh. 4 - Prob. 53TQCh. 4 - Prob. 54TQCh. 4 - Prob. 55TQCh. 4 - Prob. 56TQCh. 4 - Prob. 57TQCh. 4 - Prob. 58TQCh. 4 - Prob. 59TQCh. 4 - Prob. 60TQCh. 4 - Prob. 61TQCh. 4 - Prob. 62TQ
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