Principles of Biology
Principles of Biology
2nd Edition
ISBN: 9781259875120
Author: Robert Brooker, Eric P. Widmaier Dr., Linda Graham Dr. Ph.D., Peter Stiling Dr. Ph.D.
Publisher: McGraw-Hill Education
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Chapter 39.6, Problem 2TYK
Summary Introduction

Introduction:

A contraceptive is a drug or method or device that helps prevent pregnancy. It interferes with the normal method of ovulation, fertilization, and implantation to prevent pregnancy. It is also termed as birth control. Methods of contraceptives can be either temporary or permanent. 

  • Temporary Method: It includes the barrier method, which prevents sperm from reaching an egg. Temporary methods are condoms, vaginal diaphragms, birth control pills, intrauterine devices (IUDs), etc.
  • Permanent Method: It includes a surgical process that permanently prevents the transport of sperm through the reproductive tract. Permanent methods are tubal ligation (in women) and vasectomy (in man).

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
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