Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 39, Problem 4Q
Are any organisms other than the culture assigned for this experiment growing on the plates? How can you tell?
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Noah wanted to transfer Staphylococcus aureus from a broth to an agar plate. He picked up the broth culture, removed the cap, and flamed the mouth of the tube. He inserted an inoculating loop to obtain a bacterial sample. Then, he flamed the mouth of the tube and replaced the cap. Noah opened the lid of a labeled agar plate diagonally and used the loop to streak the surface of the agar. After closing the lid, he flamed the loop in an incinerator and put it back in its container. The plate was incubated upside down for 24–48 hours. What did Noah do wrong in this transfer? a. He did not use the transfer tool correctly. b. He did not handle the culture tube correctly. c. He did not handle the agar plate correctly. d. He used the wrong tool in transfer.
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Chapter 39 Solutions
Laboratory Experiments in Microbiology (11th Edition)
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