Why does fixation of the positive dye help bacteria stick to the slides?
Q: What is the purpose of the alcohol (OH) in the gram staining process?
A: Usually, microbes are colorless, so it’s very difficult to see them even after magnifying under…
Q: Why is heat necessary in spore staining?
A: BASIC INFORMATION SPORULATION It is a process during which spores are formed. We can find it in…
Q: What is the correct shape of the unknown bacterium growing on your MacConkey agar?
A: Macconkey Agar This agar includes bile salts, which are found in the gut. Bile salts help in…
Q: Why is the Gram stain considered a differential stain?
A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…
Q: in the clinic, the acid-fast stain is used to diagnose infection with which bacterium?
A: The Ziehl-Neelson (ZN) stain is the most commonly utilized of the different types of AF staining…
Q: why do some microbiologist use soil for DNA extraction
A: The DNA is exceedingly rare in the soil compared with that from plants, animals, fungi and microbes…
Q: Do we have to do an antibiotic sensitivity test to ensure the result? and what is the type and name…
A: Antibiotic Sensitivity Test: Antibiotic sensitivity testing, often known as antibiotic…
Q: Why is it important for a microbiology laboratory technician to use aseptic technique?
A: The majority of the microbes onto which scientists work in laboratories are known pathogens.
Q: Is the flagellar stain a Positive or Negative Simple Stain protocol?
A: Staining is a technique that is used to enhance contrast in samples, generally at the microscopic…
Q: shape
A: There are different shapes of bacteria and different classes of bacteria on its shape These shapes…
Q: If you were working with an unlabeled simple stained smear, would you be able to identify the…
A: Staining is used to colour biological specimen such as cell to identify them. Simple staining…
Q: Which bacterial morphology is demonstrated by this simple stain? choose as many as are correct
A: For all types of bacterial cells, a simple staining method may be used to give contrast to the…
Q: What is the purpose of the quadrant streak plate method? - How could this method be used in a…
A: In quadrant streak plate method, first of all the plate on which bacteria has to be grown that is on…
Q: In gram staining, if bacteria retain the primary stain after decolorizing agent treatment. Then the…
A: Answer: GRAM STAINING = It is the differential staining method to differentiate between gram…
Q: Why do serial dilution? Does serial dilution make it easier to count bacteria on a spread/streak…
A: Dilution is the process of making a solution weaker or less concentrated.
Q: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…
A: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…
Q: Why did the soap have to “cure” in the molds?
A: Fats and oils are triglycerides having a glycerol backbone with all the hydroxyl groups esterified…
Q: Why is it important to follow sterile technique?
A: Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: Why might viable cell cultures be of more use in microbialtaxonomy than preserved specimens?
A: Microbial taxonomy is a field of biological sciences that deals with the grouping of microorganisms…
Q: Why is nigrosin-water- bacteria puddle need to be mixed in a Simple/Negative staining
A: Staining is a technique used in slide preparation to color the specimen. With light microscopy,…
Q: Why can't you see the bacteria when you first swab a plate
A: Bacteria are one-celled organisms that make up a vast group. They can dwell in a variety of…
Q: what is gram staining
A: Bacteria is a vast group of microorganisms that consists of various shapes, sizes, structure and…
Q: Is it likely that a pure culture could be isolated from the human body? Why or why not?
A: In microbiology the pure culture is a laboratory culture that contains a single species of organism.…
Q: How would the slide appear if the bacteria were stained with safranin, a basic red dye?
A: The appearance of bacteria, when stained with safranin, is to be determined.
Q: About the Gram staining technique, explain: What is the purpose of using lugol and alcohol in this…
A: Gram staining technique is a differential test based on the composition of bacteria cell wall. It…
Q: What is the main advantage of using the slide culture technique for identifying molds?
A: Side Culture - It is a rapid method of preparing fungal colonies for examination and identification.…
Q: Why are smears of capsulated bacteria not heat-fixed before staining as opposed to Gram staining?
A: Gram staining is a differential staining technique developed by Danish bateriologist Hans Christian…
Q: Why must young cultures be used when doing a Gram stain?
A: Gram staining is a common technique that is used to differentiate bacteria into two broad groups…
Q: Based on the photo, describe the bacteria observed from the different staining technique. -…
A: Microbiology was a blessing in the eradication of mass-destructive epidemics such as rabies,…
Q: What was the purpose of leaving the cover on one set of agar plates? How did the growth on these…
A: The only microorganisms that can grow anywhere are bacteria. They can grow on specially prepared…
Q: Why is it called as differential stain
A: Staining is a technique used in slide preparation to color the specimen.With light microscopy,…
Q: List the step-by-step procedure of Gram staining.
A: Gram staining, established by Danish Microbiologist Hans Christian Gram in 1884, is by far the most…
Q: Which bacterial species exhibited true motility on the slides?
A: Bacteria are single-celled prokaryotes ubiquitous in nature. As such, they can be found in different…
Q: After performing a Gram stain on a Gram negative bacteria, the specimen does not show pink or purple…
A: steps that are included in the Gram staining are- applying a primary stain (crystal violet) to a…
Q: Why do we need to stain microorganisms?
A: Microbiology is the study of microbes. Microorganisms include bacteria, fungus, archaea, and…
Q: how to find out the test is differential or selective in the microbiology lab test?
A: Many kinds of medias are used in microbial study. Two types of media having similar inferred names…
Q: How do we reconcile the number of colonies in the plates to the actual number of bacteria in the…
A: Microbes may enter the body in a variety of ways and cause infection everywhere, but antigen and…
Q: What is the advantage of gram staining over simple staining
A:
Q: What is the purpose of iodine in the gram staining process? Explain.
A: Gram stains are used to distinguish between bacteria with and without cell walls. When the gramme…
Q: Why can't gram staining be used for mycobacterium?
A: Gram staining is one of the staining techniques. This is usually used for differentiating between…
Q: why is gram staining important
A: Gram staining is a laboratory technique which is used to differentiate between different types of…
Q: Why sometimes specimen may show organisms under the microscope but not appear in the culture media?
A: Introduction We are surrounded by various pathogens such as bacteria, viruses, fungus etc. Bacteria…
Q: Why does nigrosin not stain the bacteria?
A: Nigrosin is an acidic stain, used in the negative staining technique. Staining is a part of…
Q: Are there any chemical differences between the cell walls of gram-positive and negative bacteria…
A: Bacteria are the prokaryotes which fall under Kingdom Monera. They can be found everywhere. They…
Q: How can the thickness of the smear affect the staining procedure?
A: Smear can e defined as the spread of cells in a layer to observe it under microscope. Since, cells…
Q: Does the gram staining method still have a place in today's world of advanced diagnostic methods? If…
A: Gram staining is a type of staining which is used to differentiate bacteria on the basis of…
Q: Specific Dyes used in flagella stain: A wet-mount procedure (Ryu method) Dried-smear preparation…
A: Flagella is a hair-like structure that is found in bacteria, archaea, and Eukaryota. It allows the…
Q: What staining method would you use to verify that the isolate is not producing survival structures…
A: Staining is the procedure that helps to visualize the minute organisms under a microscope using…
Q: What is the goal of isolation streak plate technique? Why is microbial considered a pure culture?
A: Streaking : Streaking for isolation on an agar plate involves the successive dilution of organisms…
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- What general type of growth medium would you use to: (a) grow one type of bacteria but inhibit the growth of another type? (b) discriminate between different types of bacteria?Why does a one-step growth curve differ in shapefrom that of a bacterial growth curve?Bacteria grow in a nutrient solution at a rate proportional to the amount present. Initially, there are 250strands of the bacteria in the solution which grows to 800 strands after seven hours. Show (a) anexpression for the approximate number of strands in the culture at any time t and (b) the time needed forthe bacteria to grow to 1600 strands.
- Which bacterial type ( Gram positive or Gram negative) is more sensitive to the antibiotic penicillin? Explain why?Why do acid-fast positive bacteria grow more slowly?What cell types would be able to grow on the ECM in the following situations? F- , Hfr , Mix a) Streptomycin was not added to the ECM. b) The ECM contains thiamine. c) The ECM contains all 20 amino acids and all 5 nitrogenous nucleic acid bases
- Clostridium tetani is a common soil bacterium and causes tetanus. Would you expect C. tetani to possess the enzyme catalase? Explain.Why does bacterial growth stop temporarily when the glucose supply in the medium is used up?Why are the streak plates for microorganisms in coconut water incubated at 7°C?
- What would be added to a flask with E.coli incubated in the presence of oxygen to allow the maximum amount of growth? And what would be added to flasks including E.coli incubated in the absence on oxygen to allow maximum amount of growth?Escherichia coli but not Pyrolobus fumarii will grow at 40°C,while P. fumarii but not E. coli will grow at 110°C. What ishappening (or not happening) to prevent growth of eachorganism at the nonpermissive temperature?Explain why a green metallic sheen is formed when E. coli is grown on Eosin Methylene Blue Agar.