BIOLOGY
5th Edition
ISBN: 9781265202859
Author: BROOKER
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Chapter 38.1, Problem 2CC
Summary Introduction
To explain: The process of chlorosis with respect to the iron deficiency in plants.
Introduction: Different combination of elements is required for the production of chlorophyll in plants. Deficiency in either of the elements can lead to yellowing of leaves, the process is called chlorosis. Yellowing of leaves decreases the photosynthetic rate in many plants.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 38 Solutions
BIOLOGY
Ch. 38.1 - Prob. 1CCCh. 38.1 - Prob. 2CCCh. 38.2 - Prob. 1CCCh. 38.2 - How might soil crusts influence the ecology and...Ch. 38.2 - Prob. 3CCCh. 38.2 - Prob. 1EQCh. 38.2 - Prob. 2EQCh. 38.3 - Prob. 1CSCh. 38.3 - Prob. 2CSCh. 38 - Which of the following substances can limit plant...
Ch. 38 - In what form do plants take up most soil minerals?...Ch. 38 - Prob. 3TYCh. 38 - Soil organic matter provides the benefit of a....Ch. 38 - Prob. 5TYCh. 38 - Which property is not characteristic of clay-rich...Ch. 38 - Prob. 7TYCh. 38 - Prob. 8TYCh. 38 - Prob. 9TYCh. 38 - Which plant uses a passive trap to obtain animal...Ch. 38 - Prob. 1CQCh. 38 - Prob. 2CQCh. 38 - Prob. 3CQCh. 38 - Prob. 1COQCh. 38 - Prob. 2COQ
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