Campbell Biology In Focus, Loose-leaf Edition (3rd Edition)
3rd Edition
ISBN: 9780134895727
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky
Publisher: PEARSON
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Textbook Question
Chapter 37.4, Problem 3CC
MAKE CONNECTIONS Name one or more membrane activities that occur both in fertilization of an egg and in neurotransmission across a synapse (see Figure 36.14).
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 37 Solutions
Campbell Biology In Focus, Loose-leaf Edition (3rd Edition)
Ch. 37.1 - Prob. 1CCCh. 37.1 - Describe the basic pathway of information flow...Ch. 37.1 - WHAT IF? How might increased branching of an axon...Ch. 37.2 - Under what circumstances could ions flow through...Ch. 37.2 - WHAT IF? Suppose a cells membrane potential shifts...Ch. 37.2 - Prob. 3CCCh. 37.3 - How do action potentials and graded potentials...Ch. 37.3 - In multiple sclerosis (from the Greek skleros,...Ch. 37.3 - Prob. 3CCCh. 37.3 - WHAT IF? Suppose a mutation caused gated sodium...
Ch. 37.4 - Prob. 1CCCh. 37.4 - Organophosphate pesticides work by inhibiting...Ch. 37.4 - MAKE CONNECTIONS Name one or more membrane...Ch. 37 - What happens when a resting neuron's membrane...Ch. 37 - Prob. 2TYUCh. 37 - Prob. 3TYUCh. 37 - Why are action potentials usually conducted in one...Ch. 37 - Which of the following is a direct result of...Ch. 37 - Suppose a particular neurotransmitter causes an...Ch. 37 - Prob. 7TYUCh. 37 - Prob. 8TYUCh. 37 - DRAW IT Suppose a researcher inserts a pair of...Ch. 37 - Prob. 10TYUCh. 37 - FOCUS ON EVOLUTION An action potential is an...Ch. 37 - Prob. 12TYUCh. 37 - SYNTHESIZE YOUR KNOWLEDGE The rattlesnake alerts...
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