PRESCOTT'S MICROBIOLOGY
11th Edition
ISBN: 2818440045677
Author: WILLEY
Publisher: MCG
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Textbook Question
Chapter 37, Problem 1AL
ELISA tests usually use a primary and secondary antibody. Why? What are the necessary controls one would need to perform to ensure that the antibody specificities are valid (i.e., no false-positive or false-negative reactions)?
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Chapter 37 Solutions
PRESCOTT'S MICROBIOLOGY
Ch. 37.2 - Prob. 1MICh. 37.2 - Prob. 2MICh. 37.2 - Prob. 1CCCh. 37.2 - Describe in general how biochemical tests are used...Ch. 37.2 - Prob. 3CCCh. 37.2 - Prob. 4CCCh. 37.2 - Prob. 5CCCh. 37.2 - Prob. 6CCCh. 37.3 - Prob. 1MICh. 37.3 - Prob. 2MI
Ch. 37.3 - Prob. 1CCCh. 37.3 - Prob. 2CCCh. 37.3 - Prob. 3CCCh. 37.3 - Prob. 4CCCh. 37.3 - Prob. 5CCCh. 37.3 - Name two types of immunodiffusion tests and...Ch. 37 - Prob. 1RCCh. 37 - Prob. 2RCCh. 37 - Prob. 3RCCh. 37 - Prob. 4RCCh. 37 - ELISA tests usually use a primary and secondary...Ch. 37 - Prob. 2ALCh. 37 - Prob. 3AL
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- What is the difference between an indirect ELISA and a sandwich ELISA? Is rapid antibody testing a form of ELISA?arrow_forwardWhat are the advantages and disadvantages of agglutinationtests versus fluorescent antibody assays? How are the latter usedto identify specific cells in complex mixtures, such as blood?arrow_forwardIn this diagram, why is anti-human IgG used in the indirect test but not in the direct test?arrow_forward
- If you were using the ELISA to look for the presence of antibodies and the sample gave a negative result, does this mean that there were no antibodies present? Explain.arrow_forwardWhat are the two fluorescent compounds that can be used in antibody labelling?arrow_forwardWhy do some tests for antibody in serum (such as for HIV and syphilis)require backup verification with additional tests at a later date?arrow_forward
- A. Prepare a diagram with labels showing the similarities and differences among the four major types of ELISA based on their capture and detection systems. A1. Give at least one clinical application for each type of ELISA. B. What is the purpose of adding horseradish peroxidase, alkaline phosphatase, and their respective substrates?arrow_forwardCan blood typing be performed by reacting anti-body group antibodies with serum? Explain why or why not.arrow_forwardWhat is a secondary antibody in an ELISA test?arrow_forward
- Give Explanation Solutionarrow_forwardObserve figure and make note of the several steps in theindirect ELISA test. What four essential events are necessary todevelop a positive reaction (besides having antibody A)? Hint: Whatwould happen without rinses?arrow_forwardwhich of the following are necessary to run a valid elisa test?arrow_forward
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