PRESCOTT'S MICROBIOLOGY
11th Edition
ISBN: 2818440045677
Author: WILLEY
Publisher: MCG
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Chapter 37.2, Problem 2MI
Summary Introduction
To determine: The type of immunofluorescence which would be most likely to be used to test a clinical sample for the presence of an intracellular pathogen such as hepatitis C virus.
Introduction: ELISA which is also called Enzyme-Linked immune-sorbent Assay is the technique in which an enzyme-linked indicator antibody is used to visualize antigen-antibody reactions. Indirect and direct immune-sorbent assay are the two types of ELISA. The indirect immune-sorbent assay detects antibodies while direct immune-sorbent assay detects antigens.
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Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario.
Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote?
You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab.
What is one experiment you could do, involving culturing the organism?
Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario.
Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote?
You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab.
What is a procedure you could do, involving making a slide of the organism?
What are the four steps of an ELISA (enzyme-linked immunosorbent assay) protocol?
Chapter 37 Solutions
PRESCOTT'S MICROBIOLOGY
Ch. 37.2 - Prob. 1MICh. 37.2 - Prob. 2MICh. 37.2 - Prob. 1CCCh. 37.2 - Describe in general how biochemical tests are used...Ch. 37.2 - Prob. 3CCCh. 37.2 - Prob. 4CCCh. 37.2 - Prob. 5CCCh. 37.2 - Prob. 6CCCh. 37.3 - Prob. 1MICh. 37.3 - Prob. 2MI
Ch. 37.3 - Prob. 1CCCh. 37.3 - Prob. 2CCCh. 37.3 - Prob. 3CCCh. 37.3 - Prob. 4CCCh. 37.3 - Prob. 5CCCh. 37.3 - Name two types of immunodiffusion tests and...Ch. 37 - Prob. 1RCCh. 37 - Prob. 2RCCh. 37 - Prob. 3RCCh. 37 - Prob. 4RCCh. 37 - ELISA tests usually use a primary and secondary...Ch. 37 - Prob. 2ALCh. 37 - Prob. 3AL
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