Biology
Biology
12th Edition
ISBN: 9780078024269
Author: Sylvia Mader, Michael Windelspecht
Publisher: McGraw-Hill Education
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Chapter 34, Problem 2A
Summary Introduction

Introduction:

Typhlosole is the internal folding of skin present in the intestinal cavity of the earthworms. They are folds of the intestine, which help in the digestion, absorption and assimilation of food. It can be compared to the folds called as villi present in the intestine.

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If PCR was performed on the fragment of DNA shown below using "5'-TAGG-3" and "3'-TCTA-5'" as the primers, how many base pairs long would the PCR product be? To help with this, remember the antiparallel structure of DNA and that primers are complementary and antiparallel to the target sequence that they bind to. Hint: Check out the 5' and 3' labels....they are important! 3’- T  A  T  C  C  G  A  C  A  A  T  C  G  A  T  C  G  A  T  T  G  C  C  T  T  C  T  A  A -5’ 5’- A  T  A  G  G  C  T  G  T  T  A  G  C  T  A  G  C  T  A  A  C  G  G  A  A  G A  T  T – 3’
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