Campbell Biology: Australian And New Zealand Edition + Mastering Biology With Etext
11th Edition
ISBN: 9781488687075
Author: Lisa, A. Urry
Publisher: PEARSON
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Chapter 33.4, Problem 1CC
Summary Introduction
To differentiate: Between the body plan of nematodes and annelids.
Concept introduction:
The basic difference in the body plan of nematode and annelid is that the annelids are eucoelomate (having true coelom). On the other hand, the nematodes are pseudocoelomates (having false coelom). The annelids show true segmentation unlike nematodes. The nematodes are mostly parasitic. On the other hand, only some annelids are parasitic.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 33 Solutions
Campbell Biology: Australian And New Zealand Edition + Mastering Biology With Etext
Ch. 33.1 - Prob. 1CCCh. 33.1 - Prob. 2CCCh. 33.2 - Compare and contrast the polyp and medusa forms of...Ch. 33.2 - VISUAL SKILLS Use the cnidarian life cycle...Ch. 33.2 - MAKE CONNECTIONS Many new animal body plans...Ch. 33.3 - Prob. 1CCCh. 33.3 - Prob. 2CCCh. 33.3 - MAKE CONNECTIONS Explain how the molluscan foot...Ch. 33.4 - Prob. 1CCCh. 33.4 - Describe two adaptations that have enabled insects...
Ch. 33.4 - MAKE CONNECTIONS Historically, annelids and...Ch. 33.5 - Prob. 1CCCh. 33.5 - WHAT IF? The insect Drosophila melanogaster and...Ch. 33.5 - MAKE CONNECTIONS Describe how the features and...Ch. 33 - Lacking tissues and organs, how do soonges...Ch. 33 - Describe the cnidarian body plan and its two major...Ch. 33 - is the lophotrochozoan clade united by unique...Ch. 33 - Describe some ecological roles of nematodes and...Ch. 33 - You've read that echinoderms and chordates are...Ch. 33 - A land snail, a clam and an octopus all share...Ch. 33 - Prob. 2TYUCh. 33 - The water vascular system of echinoderms (A)...Ch. 33 - Prob. 4TYUCh. 33 - In Figure 33.2, which two main clades branch from...Ch. 33 - MAKE CONNECTIONS In Figure 33.8, assume that the...Ch. 33 - Evolution connection Interpret thf data Draw a...Ch. 33 - Prob. 8TYUCh. 33 - Prob. 9TYUCh. 33 - SYNTHESIZE YOUR KNOWLEDGE Colleclively, do these...
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