Campbell Biology: Australian And New Zealand Edition + Mastering Biology With Etext
11th Edition
ISBN: 9781488687075
Author: Lisa, A. Urry
Publisher: PEARSON
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Chapter 33, Problem 6TYU
MAKE CONNECTIONS In Figure 33.8, assume that the two medusae shown ai step 4 were produced by one polyp colony. Review Concept 12.1 and Concept 13.3, and then use your understandingof mitosisand meiosis to evaluate whether the following sentence is Irue oi false; if false, seiet t the am wer that providcs the correct reason. Although the two medusae are genetically identical, a sperm produced by one will differ genetically front an egg produced by the other.
- (A) False {both the medusae and the gametes are genetically identical)
- (B) False (neither the medusae nor the gametes are genetically identical)
(C) False {the medusae are not identical but the gametes are)
(D) True
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 33 Solutions
Campbell Biology: Australian And New Zealand Edition + Mastering Biology With Etext
Ch. 33.1 - Prob. 1CCCh. 33.1 - Prob. 2CCCh. 33.2 - Compare and contrast the polyp and medusa forms of...Ch. 33.2 - VISUAL SKILLS Use the cnidarian life cycle...Ch. 33.2 - MAKE CONNECTIONS Many new animal body plans...Ch. 33.3 - Prob. 1CCCh. 33.3 - Prob. 2CCCh. 33.3 - MAKE CONNECTIONS Explain how the molluscan foot...Ch. 33.4 - Prob. 1CCCh. 33.4 - Describe two adaptations that have enabled insects...
Ch. 33.4 - MAKE CONNECTIONS Historically, annelids and...Ch. 33.5 - Prob. 1CCCh. 33.5 - WHAT IF? The insect Drosophila melanogaster and...Ch. 33.5 - MAKE CONNECTIONS Describe how the features and...Ch. 33 - Lacking tissues and organs, how do soonges...Ch. 33 - Describe the cnidarian body plan and its two major...Ch. 33 - is the lophotrochozoan clade united by unique...Ch. 33 - Describe some ecological roles of nematodes and...Ch. 33 - You've read that echinoderms and chordates are...Ch. 33 - A land snail, a clam and an octopus all share...Ch. 33 - Prob. 2TYUCh. 33 - The water vascular system of echinoderms (A)...Ch. 33 - Prob. 4TYUCh. 33 - In Figure 33.2, which two main clades branch from...Ch. 33 - MAKE CONNECTIONS In Figure 33.8, assume that the...Ch. 33 - Evolution connection Interpret thf data Draw a...Ch. 33 - Prob. 8TYUCh. 33 - Prob. 9TYUCh. 33 - SYNTHESIZE YOUR KNOWLEDGE Colleclively, do these...
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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