EBK HUMAN ANATOMY & PHYSIOLOGY
1st Edition
ISBN: 9780100659834
Author: AMERMAN
Publisher: YUZU
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Textbook Question
Chapter 3.3, Problem 8QC
Describe the process of secondary active transport.
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 3 Solutions
EBK HUMAN ANATOMY & PHYSIOLOGY
Ch. 3.1 - What are the general functions of cells?
Ch. 3.1 - Prob. 2QCCh. 3.1 - Where are intracellular and extracellular fluids...Ch. 3.2 - How do the phospholipids arrange themselves in the...Ch. 3.2 - 2. How is the plasma membrane described according...Ch. 3.2 - List five functions of membrane proteins.Ch. 3.2 - What roles do cholesterol, glycoproteins, and...Ch. 3.3 - The energy for passive processes comes from the...Ch. 3.3 - Mark each of the following statements as true or...Ch. 3.3 - Simple diffusion is a passive process, but...
Ch. 3.3 - Mark each of the following statements as true or...Ch. 3.3 - Mark each of the following statements as true or...Ch. 3.3 - How does the process of primary active transport...Ch. 3.3 - What is the main primary active transport pump in...Ch. 3.3 - Describe the process of secondary active...Ch. 3.3 - What are the three types of endocytosis, and how...Ch. 3.3 - Explain the basic process of exocytosis.Ch. 3.4 - 1. Identify the properties listed in the next...Ch. 3.4 - Identify the following properties as belonging to...Ch. 3.4 - To what destinations can products from the Golgi...Ch. 3.5 - Prob. 1QCCh. 3.5 - Prob. 2QCCh. 3.5 - Prob. 3QCCh. 3.6 - 1. What are the main components of the nucleus?...Ch. 3.6 - What is chromatin? How are chromatin and...Ch. 3.6 - 3. What is a nucleolus, and what is its...Ch. 3.7 - How is a codon related to a triplet?Ch. 3.7 - 2. Describe the basic steps of transcription.
Ch. 3.7 - Explain how tRNA acts as the translator of the...Ch. 3.7 - Describe the basic steps of translation.Ch. 3.7 - 5. Why is posttranslational modification...Ch. 3.7 - 6. Why is it important to regulate gene...Ch. 3.8 - What happens during each stage of the cell cycle?Ch. 3.8 - What does semiconservative replication mean?Ch. 3.8 - Describe the changes in the cell that take place...Ch. 3.8 - What are four external factors that play a role in...Ch. 3 - Which of the following is not a basic function...Ch. 3 - Prob. 2CYRCh. 3 - What are the two fluid compartments in the body,...Ch. 3 - 4. Which of the following best describes the...Ch. 3 - Mark the following statements about the plasma...Ch. 3 - 6. What is the primary difference between active...Ch. 3 - 7. Match the term with its appropriate...Ch. 3 - 8. Fill in the blanks: A hypotonic solution will...Ch. 3 - 9. Match the following terms with the correct...Ch. 3 - Prob. 10CYRCh. 3 - Mark the following statements about the...Ch. 3 - 12. Our somatic cells’ DNA is distributed among...Ch. 3 - Explain how and why chromatin is condensed in the...Ch. 3 - Which of the following statements correctly...Ch. 3 - Each of the following statements about protein...Ch. 3 - Number the following steps of protein synthesis in...Ch. 3 - Which of the following is not a phase of mitosis?...Ch. 3 - 18. Why is regulation of the cell cycle...Ch. 3 - 19. Mark the following statements about the cell...Ch. 3 - 20. Match the following terms with the correct...Ch. 3 - 1. Write a single sentence, using no more than 25...Ch. 3 - 2. Certain diseases are transmitted via...Ch. 3 - 3. Explain how the form of each of the following...Ch. 3 - Certain types of cancerous lung tumors can secrete...Ch. 3 - Why do you think the rate of cell division is...Ch. 3 - 1. A patient is admitted to the hospital and...Ch. 3 - A popular science fiction program once had an...Ch. 3 - Prob. 3AYKACh. 3 - Prob. 4AYKACh. 3 - 5. Explain how buffer systems in the body work if...
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forwardWhich of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forward
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