Biological Science, Books a la Carte Plus Mastering Biology with Pearson eText -- Access Card Package (6th Edition)
Biological Science, Books a la Carte Plus Mastering Biology with Pearson eText -- Access Card Package (6th Edition)
6th Edition
ISBN: 9780134296029
Author: Scott Freeman, Kim Quillin, Lizabeth Allison, Michael Black, Greg Podgorski, Emily Taylor, Jeff Carmichael
Publisher: PEARSON
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Chapter 33, Problem 12PIAT
Summary Introduction

To review:

The results of the HIV (human immunodeficiency virus) experiment and compare them with the Ebola virus (EBOV) experiment.

Introduction:

Ebola virus can lead to severe and fatal hemorrhagic fever in the human beings. The disease caused is known as Ebola virus disease. HIV is the subgroup of retrovirus (lentivirus) that leads to AIDS (acquired immunodeficiency syndrome). HIV utilizes CD4 (cluster of differentiation 4) receptor in order to enter the T helper cells. The cells that have free and unbound CD4 on their surface will be infected by the HIV. CD4 is a membrane protein that HIV utilizes to enter the cells. Similarly, researchers have identified many surface proteins that may work as a virus receptor for the EBOV. The figure below shows the experiment that was performed using HIV virus.

Biological Science, Books a la Carte Plus Mastering Biology with Pearson eText -- Access Card Package (6th Edition), Chapter 33, Problem 12PIAT

Experiment: One hundred sixty identical samples of T helper cells were grown in the culture. Different antibodies were then added into each sample of the cells. The antibody inhibits the specific membrane protein. A constant number of virions were then added to the samples followed by the incubation process under the conditions that are optimal for the viruses to enter.

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After you feel comfortable with your counting method and identifying cells in the various stages of mitosis, use the four images below of whitefish blastula to count the cells in each stage until you reach 100 total cells, recording your data below in Data Table 1. (You may not need to use all four images. Stop counting when you reach 100 total cells.) After totaling the cells in each stage, calculate the percent of cells in each stage. (Divide total of stage by overall total of 100 and then multiply by 100 to obtain percentage.)   Data Table 1Stage    Totals    PercentInterphase        Mitosis:        Prophase        Metaphase        Anaphase        Telophase        Cytokinesis        Totals    100    100% To find the length of time whitefish blastula cells spend in each stage, multiply the percent (recorded as a decimal, in other words take the percent number and divide by 100) by 24 hours. (Example: If percent is 20%, then Time in Hours = .2 * 24 = 4.8) Record your data in Data…
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