Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 32, Problem 4P
Interpretation Introduction

Interpretation:

The two important ways in which the lac repressor and pur repressor differences can be determined.

Concept introduction:

E. Coli contains of lac operon, which is involved in lactose metabolism. It can be expressed in the presence of lactose and absence of glucose. The two regulators of lac operons are the lac repressor and the CAP. The function of these two regulators is to turn ‘on’ and ‘off’ the operon in response to glucose and lactose.

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a) What are the differences between the Direct & Indirect Immunofluorescence Assays? (0.5 mark) b) What are the advantages of the Indirect Immunofluorescence Assays? (0.5 mark) c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality? (2 marks)
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