Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 32, Problem 2P
Interpretation Introduction
Interpretation:
The concentration of lac repressor should be calculated.
The free or bound state of lac repressor with DNA should be determined.
Concept introduction:
E. Coli contains of lac operon, which is involved in lactose
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Draw a tripeptide of your choosing at pH 7. Have the N-terminus on the left and the C-terminus on the right. Then:
Draw a triangle around the α-carbons.
Draw a box around the R-groups.
Circle the atoms capable of hydrogen bonding.
Highlight the atoms involved in the formation of the peptide bonds.
What type of structure have you drawn? (primary, secondary, tertiary or quaternary protein structure).
2. For the flow of fluid over a flat membrane of length 10cm,
determine the length-average mass transfer coefficient. The
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Background
Freezing isn't the only challenge in cryopreservation - thawing can
be just as difficult. A microwave oven seems like a nice solution,
since it deposits energy quickly and microwaves are non-ionizing
radiation (they do not cause DNA mutation). However, water
absorbs microwaves more effectively than ice does, meaning that
the portion of an organ that has already melted will get warmer at a
higher rate than the remaining ice – the opposite of what we want!
-
The transmission of radiation through a weakly absorbing material
such as ice or water can be modeled by Beer's law, which assumes
that the rate of absorption at a depth x is proportional to the local
radiation intensity I(x) times an absorption coefficient, which is often
written as μ or a or just µ). Noting that absorption decreases the
intensity, we can write a differential equation
a
Solving the differential equation with the boundary condition on the
surface being
gives the relationship
For a standard microwave oven…
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