Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 32, Problem 2P
Interpretation Introduction
Interpretation:
The concentration of lac repressor should be calculated.
The free or bound state of lac repressor with DNA should be determined.
Concept introduction:
E. Coli contains of lac operon, which is involved in lactose
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Please draw out the molecular structures of each molecule and show how each enzyme + cofactor would affect the following molecule in the human metabolic pathway.
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- B- Vitamins are converted readily into important metabolic cofactors. Deficiency in any one of them has serious side effects. a. The disease beriberi results from a vitamin B 1 (Thiamine) deficiency and is characterized by cardiac and neurological symptoms. One key diagnostic for this disease is an increased level of pyruvate and α-ketoglutarate in the bloodstream. How does this vitamin deficiency lead to increased serumlevels of these factors? b. What would you expect the effect on the TCA intermediates for a patient suffering from vitamin B 5 deficiency? c. What would you expect the effect on the TCA intermediates for a patientsuffering from vitamin B 2 /B 3 deficiency?arrow_forwardPyruvate is accepted into the TCA cycle by a “feeder” reaction using the pyruvate dehydrogenase complex, resulting in acetyl-CoA and CO2. Provide a full mechanism for this reaction utilizing the TPP cofactor. Include the roles of all cofactors.arrow_forwardMap out all of the metabolic pathways in the liver cell. Draw out the structures and names of all compounds neatly by hand and the pathways responsible for metabolizing them. Some examples are: Glycolysis/gluconeogenesis, PPP, Glycogenesis/glycogenolysis, Krebs, ETC, selectamino acid pathways (Ala, Glu, Asp) Lipogenesis/lipolysis. Citrate/MAS/glycerol phosphate shuttlesystems, and the Cori/Glc-Ala cycles. Rules:-Draw both a mitochondrial area of metabolism and a cytoplasmic area of metabolism.-Draw the liver and its roles in glucose recycling (Cori cycle/Glc-Alanine recycling)-Avoid drawing the same molecule twice (except for separate mitochondrial/cytoplasmic populations. i.e. Design the PPP/Glycolysis so that GAP is only drawn once)-Label Carbon 4 of glucose and highlight where you would expect to find it in EVERY compound in whichit is present.-Have one or two locations for NADH/NADPH/ATP/GTP/CoQH2 – many arrows will come to/from thesespots.arrow_forward
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