Campbell Biology In Focus, Loose-leaf Edition (3rd Edition)
3rd Edition
ISBN: 9780134895727
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky
Publisher: PEARSON
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Chapter 32, Problem 2TYU
Summary Introduction
Introduction:
The rate of the exchange of heat between an animal and its environment is called as the phenomenon of “Thermoregulation”. These animals generate heat and maintain the temperature within the environment by a flow of air over the body surface. The heat gets exchanged between the blood vessels in the circulatory system and the external environment.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 32 Solutions
Campbell Biology In Focus, Loose-leaf Edition (3rd Edition)
Ch. 32.1 - What properties do all types of epithelia share?Ch. 32.1 - Prob. 2CCCh. 32.2 - Prob. 1CCCh. 32.2 - Prob. 2CCCh. 32.2 - Prob. 3CCCh. 32.3 - Is it accurate to define homeostasis as a constant...Ch. 32.3 - Prob. 2CCCh. 32.3 - Prob. 3CCCh. 32.4 - What is the function of the filtration step in...Ch. 32.4 - What advantage does uric acid offer as a...
Ch. 32.4 - WHAT IF? A camel standing in the sun requires much...Ch. 32.5 - Prob. 1CCCh. 32.5 - Prob. 2CCCh. 32.5 - WHAT IF? If blood pressure in the afferent...Ch. 32 - The body tissue that consists largely of material...Ch. 32 - Prob. 2TYUCh. 32 - Which process in the nephron is least selective?...Ch. 32 - Homeostasis typically relies on negative feedback...Ch. 32 - Prob. 5TYUCh. 32 - In which of the following species should natural...Ch. 32 - Prob. 7TYUCh. 32 - Prob. 8TYUCh. 32 - Prob. 9TYUCh. 32 - Prob. 10TYUCh. 32 - Prob. 11TYUCh. 32 - SYNTHESIZE YOUR KNOWLEDGE These macaques (Macaca...
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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