Campbell Biology in Focus, Books a la Carte Edition; Modified Mastering Biology with Pearson eText - ValuePack Access Card - for Campbell Biology in Focus (2nd Edition)
2nd Edition
ISBN: 9780134433769
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman
Publisher: PEARSON
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Textbook Question
Chapter 31.4, Problem 2CC
Chewing insects mechanically damage plants and lessen the surface area of leaves for photosynthesis. In addition, these insects make plants more vulnerable to pathogen attack. Suggest a reason why.
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True or False?
a.____If a restriction enzyme recognizes the restriction site, 5’AACGTT3’, and the enzyme cuts between the second A and the C, this will produce a “sticky end,” which is useful for cloning into a plasmid vector.
b.____The DNA molecules will travel through the agar toward the negative end of the gel rig (black wires) during electrophoresis.
c.____CRISPR was one of the first methods discovered in the 1970s for reliably amplifying gene products so that they can be sequenced and studied.
a) Polymerase Chain Reaction is a way that scientists amplify (or create billions of copies of) DNA in vitro (in a tube.)
b) Compare PCR with DNA Replication in the cell. Circle the components that are required for PCR. For the parts that are not circled, list them below and write an explanation for why they are no longer needed when DNA replication of a target gene is done in vitro.
DNA Gyrase (or Topoisomerase)
DNA polymerase
Helicase
SSBPs
Ligase
dNTPs
Primers
Sliding Clamp
Replisome
Draw the major product of this reaction. Ignore inorganic byproducts.
O
1. NaOCH2CH3, 25°C
2. PhCH2Br (1 equiv)
Chapter 31 Solutions
Campbell Biology in Focus, Books a la Carte Edition; Modified Mastering Biology with Pearson eText - ValuePack Access Card - for Campbell Biology in Focus (2nd Edition)
Ch. 31.1 - Prob. 1CCCh. 31.1 - WHAT IF? If a plant has the double mutation ctr...Ch. 31.1 - Prob. 3CCCh. 31.2 - If an enzyme in field-grown soybean leaves is most...Ch. 31.2 - WHAT IF? If a plant flowers in a controlled...Ch. 31.2 - MAKE CONNECTIONS Plants detect the quality of...Ch. 31.3 - Prob. 1CCCh. 31.3 - Prob. 2CCCh. 31.3 - Prob. 3CCCh. 31.4 - Prob. 1CC
Ch. 31.4 - Chewing insects mechanically damage plants and...Ch. 31.4 - Prob. 3CCCh. 31 - The hormone that helps plants respond to drought...Ch. 31 - Prob. 2TYUCh. 31 - Prob. 3TYUCh. 31 - Prob. 4TYUCh. 31 - Prob. 5TYUCh. 31 - Prob. 6TYUCh. 31 - DRAW IT Indicate the response of Arabidopsis to...Ch. 31 - Prob. 8TYUCh. 31 - FOCUS ON EVOLUTION As a general rule,...Ch. 31 - FOCUS ON INTERACTIONS In a short essay (100-150...Ch. 31 - Prob. 11TYU
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