The effect of an increased Cro concentration on the expression of the λ repressor gene should be determined. The effect of an increased λ repressor gene on the Cro gene expression should be determined. The effect of an increased λ repressor on the expression of lambda repressor gene should be determined. Concept introduction: The lytic cycle is the rupture of the host cell, resulting in the release of a number of viruses. Bacterial viruses undergo lytic cycle only and are termed as the virulent bacteriophages. These viruses are the cause of their host cells death. Another life cycle of bacteriophage includes a lysogenic cycle. In this cycle, the viral DNA remains in the inactivated state even after various reproduction cycles of the host bacterium. After a certain time only, the viral DNA is released from the host’s cell DNA and undergoes lytic cycle.

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I am a Biochemistry student and I am confused on how to analyze FRAP Analysis using Excel Spread Sheets. The following spread sheet has my 0 minute data listed at top and the 4 minute data listed on the bottom. Sheet: https://mnscu-my.sharepoint.com/:x:/g/personal/vi2163ss_go_minnstate_edu/EXjrCizWiXRPmpittqZA12IB8EkB5eE8iaRqj_iun-IAtg?rtime=Wo9zPHFY3Ug The formula for FRAP Analysis is: FRAP value = A (4 min sample) - A (0 min sample) over A (4 min 30 uM ascorbic acid) - A (0 min 30 uM ascorbic acid) multiplied by 30 uM and the dilution factor of 1/10

Answer 1

Question

Chapter 31, Problem 9P

Interpretation Introduction

Interpretation:

The effect of an increased Cro concentration on the expression of the λ repressor gene should be determined.

The effect of an increased λ repressor gene on the Cro gene expression should be determined.

The effect of an increased λ repressor on the expression of lambda repressor gene should be determined.

Concept introduction:

The lytic cycle is the rupture of the host cell, resulting in the release of a number of viruses. Bacterial viruses undergo lytic cycle only and are termed as the virulent bacteriophages. These viruses are the cause of their host cells death. Another life cycle of bacteriophage includes a lysogenic cycle. In this cycle, the viral DNA remains in the inactivated state even after various reproduction cycles of the host bacterium. After a certain time only, the viral DNA is released from the host’s cell DNA and undergoes lytic cycle.

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