Biochemistry
8th Edition
ISBN: 9781464126109
Author: Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr., Lubert Stryer
Publisher: W. H. Freeman
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Chapter 31, Problem 11P
Interpretation Introduction
Interpretation:
A simple assay for autoinducer activity of compounds in Vibrio fischeri should be determined.
Concept introduction:
Vibrio fischeri is a gram-negative and rod-shaped bacterium. They can survive only in seawater, but not in fresh water. Moreover, it is a bioluminescent bacterium, which makes a symbiotic relation with the bobtail squids. The bioluminescent nature of Vibrio is due to the expression of lux operon.
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You’ve isolated a protein and determined that the Native molecular weight of the holoenzyme is 160 kD using size exclusion chromatography. Analysis of this protein using SDS-PAGE revealed 2 bands, one at 100 kD and one at 30 kD.
The enzyme was found to be 0.829% NAD (by weight). What further can be said regarding the structure of the polypeptide?
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