Biochemistry
8th Edition
ISBN: 9781464126109
Author: Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr., Lubert Stryer
Publisher: W. H. Freeman
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Chapter 31, Problem 6P
Interpretation Introduction
Interpretation:
The reason for the occurrence of 20 bp inverted repeats in a DNA sequence should be determined.
Concept introduction:
The single-stranded sequence which is followed by its complement in the reverse downstream direction, is termed as an inverted repeat. The sequences between the initial sequence and the reverse complement can be of any length.
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You’ve isolated a protein and determined that the Native molecular weight of the holoenzyme is 160 kD using size exclusion chromatography. Analysis of this protein using SDS-PAGE revealed 2 bands, one at 100 kD and one at 30 kD.
The enzyme was found to be 0.829% NAD (by weight). What further can be said regarding the structure of the polypeptide?
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