Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 3.1, Problem 2TQ
Summary Introduction
To review:
The steps for the modification of cell fractionation procedure for the isolation of flagellar motors that spans the envelope from inner to outer membrane.
Introduction:
Cellular fractionation is a technique used for the separation of cellular components by conserving their functional properties. Generally, it is used for determining the location of cellular components of several biochemical processes. The cell fraction can also enable the detailed study of the constituting parts of the cell. This technique was given by Albert Claude.
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8. Transcription and Translation Practice: (Video 10-1 and 10-2)
A. Below is the sense strand of a DNA gene. Using the sense strand, create the antisense
DNA strand and label the 5' and 3' ends.
B. Use the antisense strand that you create in part A as a template to create the mRNA
transcript of the gene and label the 5' and 3' ends.
C. Translate the mRNA you produced in part B into the polypeptide sequence making sure
to follow all the rules of translation.
5'-AGCATGACTAATAGTTGTTGAGCTGTC-3' (sense strand)
4
Chapter 3 Solutions
Microbiology: An Evolving Science (Fourth Edition)
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