(a) Interpretation: The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of 32 P-labelled ATP formation from 32 PPi is to be done by ATP and 32 PPi Concept introduction: Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond. Translation is the mechanism via which mRNA is decoded and translated into a protein.

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Answer 1

Question

Chapter 31, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by ATP and ³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Interpretation Introduction

(b)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by ATP and ³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Interpretation Introduction

(c)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by isoleucine, ATP and³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds,and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

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For a Michaelis-Menten reaction, k₁-5 x 10'/M-s, k.-2 x 10%/s, and k₂-4 x 10²/s. a) Calculate the Ks and KM for this reaction. b) Does substrate binding achieve equilibrium or steady state?

Assume that an enzyme-catalyzed reaction follows the scheme shown: E+S SES →E + P k₁ = 1 x 109/M-s k-1=2.5 x 10%/s k₂ = 3.4 x 107/s What is the dissociation constant for the enzyme-substrate, K,? What is the Michaelis constant, Km, for this enzyme? What is the turnover number, Keat, for this enzyme? What is the catalytic efficiency for the enzyme? If the initial Et concentration is 0.25mM, what is Vmax?

Answer 2

Question

Chapter 31, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by ATP and ³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Interpretation Introduction

(b)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by ATP and ³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Interpretation Introduction

(c)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by isoleucine, ATP and³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds,and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

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Answer 3

Question

Chapter 31, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by ATP and ³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Interpretation Introduction

(b)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by ATP and ³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Interpretation Introduction

(c)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by isoleucine, ATP and³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds,and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Expert Solution & Answer

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Answer 4

Question

Chapter 31, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by ATP and ³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Interpretation Introduction

(b)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by ATP and ³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Interpretation Introduction

(c)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by isoleucine, ATP and³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds,and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Expert Solution & Answer

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Answer 5

Chapter 31, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by ATP and ³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Interpretation Introduction

(b)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by ATP and ³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Interpretation Introduction

(c)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by isoleucine, ATP and³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds,and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Answer 6

Chapter 31, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by ATP and ³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Answer 7

Chapter 31, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by ATP and ³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Answer 8

Interpretation Introduction

(b)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by ATP and ³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Answer 9

Interpretation Introduction

(b)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by ATP and ³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Answer 10

Interpretation Introduction

(c)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by isoleucine, ATP and³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds,and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Answer 11

Interpretation Introduction

(c)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by isoleucine, ATP and³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds,and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Answer 12

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Answer 13

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Answer 14

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Answer 15

Ch. 31 - Prob. 1P Ch. 31 - Prob. 2P Ch. 31 - Prob. 3P Ch. 31 - Prob. 4P Ch. 31 - Prob. 5P Ch. 31 - Prob. 6P Ch. 31 - Prob. 7P Ch. 31 - Prob. 8P Ch. 31 - Prob. 9P Ch. 31 - Prob. 10P

Solution Summary: The author explains that isoleucyl-tRNA formation proceeds through the reversible formation of the enzyme bound Ile AMP intermediate.

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Chapter 31 Solutions