(a) Interpretation: The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of 32 P-labelled ATP formation from 32 PPi is to be done by ATP and 32 PPi Concept introduction: Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond. Translation is the mechanism via which mRNA is decoded and translated into a protein.

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Answer 1

Question

Chapter 31, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by ATP and ³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Interpretation Introduction

(b)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by ATP and ³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Interpretation Introduction

(c)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by isoleucine, ATP and³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds,and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

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a) What are the differences between the Direct & Indirect Immunofluorescence Assays? (0.5 mark) b) What are the advantages of the Indirect Immunofluorescence Assays? (0.5 mark) c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality? (2 marks)

a) What are the differences between the Direct & Indirect Immunofluorescence Assays? b) What are the advantages of the Indirect Immunofluorescence Assays? c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality?

Answer 2

Question

Chapter 31, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by ATP and ³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Interpretation Introduction

(b)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by ATP and ³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Interpretation Introduction

(c)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by isoleucine, ATP and³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds,and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

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Answer 3

Question

Chapter 31, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by ATP and ³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Interpretation Introduction

(b)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by ATP and ³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Interpretation Introduction

(c)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by isoleucine, ATP and³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds,and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

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Answer 4

Question

Chapter 31, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by ATP and ³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Interpretation Introduction

(b)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by ATP and ³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Interpretation Introduction

(c)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by isoleucine, ATP and³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds,and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

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Answer 5

Chapter 31, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by ATP and ³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Interpretation Introduction

(b)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by ATP and ³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Interpretation Introduction

(c)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by isoleucine, ATP and³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds,and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Answer 6

Chapter 31, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by ATP and ³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Answer 7

Chapter 31, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by ATP and ³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Answer 8

Interpretation Introduction

(b)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by ATP and ³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Answer 9

Interpretation Introduction

(b)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by ATP and ³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Answer 10

Interpretation Introduction

(c)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by isoleucine, ATP and³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds,and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Answer 11

Interpretation Introduction

(c)

Interpretation:

The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of ³²P-labelled ATP formation from ³²PPi is to be done by isoleucine, ATP and³²PPi

Concept introduction:

Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds,and the triphosphate is attached to the ribose sugar by a phosphoester bond.

Translation is the mechanism via which mRNA is decoded and translated into a protein.

Answer 12

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Answer 13

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Answer 14

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Answer 15

Ch. 31 - Prob. 1P Ch. 31 - Prob. 2P Ch. 31 - Prob. 3P Ch. 31 - Prob. 4P Ch. 31 - Prob. 5P Ch. 31 - Prob. 6P Ch. 31 - Prob. 7P Ch. 31 - Prob. 8P Ch. 31 - Prob. 9P Ch. 31 - Prob. 10P

Solution Summary: The author explains that isoleucyl-tRNA formation proceeds through the reversible formation of the enzyme bound Ile AMP intermediate.

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Chapter 31 Solutions