(a)
Interpretation:
The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of 32P-labelled ATP formation from 32PPi is to be done by ATP and 32PPi
Concept introduction:
Adenosine triphosphate or ATP is the most common
Translation is the mechanism via which mRNA is decoded and translated into a protein.
(b)
Interpretation:
The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of 32P-labelled ATP formation from 32PPi is to be done by ATP and 32PPi
Concept introduction:
Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds and the triphosphate is attached to the ribose sugar by a phosphoester bond.
Translation is the mechanism via which mRNA is decoded and translated into a protein.
(c)
Interpretation:
The formation of isoleucyl-tRNA proceeds through the reversible formation of the enzyme bound Ile AMP intermediate. Prediction of 32P-labelled ATP formation from 32PPi is to be done by isoleucine, ATP and32PPi
Concept introduction:
Adenosine triphosphate or ATP is the most common nucleotide in the cell as well as the primary cellular energy currency of in all the life forms. ATP is a type of nucleotide that has a ribose sugar, adenine nitrogenous base and a triphosphate (three-sequential phosphate group). The phosphate groups are attached to each other byphosphoanhydride bonds,and the triphosphate is attached to the ribose sugar by a phosphoester bond.
Translation is the mechanism via which mRNA is decoded and translated into a protein.

Want to see the full answer?
Check out a sample textbook solution
Chapter 31 Solutions
BIOCHEM-ACHIEVE(FIRST DAY DISCOUNTED)
- Show the fate of the hydrogen on carbon-2 of glucose.arrow_forwardImagine that aldolase can react with the seven carbon molecule Sedoheptulose-1,7-bisphosphate (below). Use the mechanism to predict the two products generated.arrow_forwardShow the mechanisms of PGK and PFK-1. How are they different?arrow_forward
- Show the fate of the proton on the 4-Oxygen molecule of F-1,6-BP.arrow_forwardSodium borohydride (NaBH4) is a potent inhibitor of aldolase. It is known to ONLY inhibit theenzyme when it is complexed with substrate. Treatment of the enzyme alone has no effect.What is the mechanism for this inhibition?arrow_forwardA non-hydrolysable ATP (AMPPNP - below) is ingested by a graduate student on a dare. Whateffects would you anticipate on his metabolism?arrow_forward
- Show the mechanism for the acid-catalyzed formation of an [α-1,6] glycosidic linkagebetween two molecules of α-D-glucopyranose. Please sketch the structure and use arrows showing electron flow!arrow_forwardI am a Biochemistry student and I am confused on how to analyze FRAP Analysis using Excel Spread Sheets. The following spread sheet has my 0 minute data listed at top and the 4 minute data listed on the bottom. Sheet: https://mnscu-my.sharepoint.com/:x:/g/personal/vi2163ss_go_minnstate_edu/EXjrCizWiXRPmpittqZA12IB8EkB5eE8iaRqj_iun-IAtg?rtime=Wo9zPHFY3Ug The formula for FRAP Analysis is: FRAP value = A (4 min sample) - A (0 min sample) over A (4 min 30 uM ascorbic acid) - A (0 min 30 uM ascorbic acid) multiplied by 30 uM and the dilution factor of 1/10arrow_forwardHO Fill in the missing boxes. ON 800 NO NO Glucose ATP NADH Hexokinase 1,3-Bisphosphoglycerate Mg2+ ADP NAD+, Pi Phosphoglucose Isomerase Glucose-6-Phosphate ON 沁 Glyceraldehyde-3-Phosphate HO حلمة ADP ADP Phospho Mg2+ glycerate Dihydroxyacetone Phosphate ATP kinase ATP Phosphoglycerate 3-phosphoglycerate Mutase H₁₂O Fructose-6-Phosphate ATP Mg2+ ADP Fructose-1,6-Bisphosphate 2-phosphoglycerate H₂O Phosphoenolpyruvate ADP Mg2+ ATP Pyruvatearrow_forward
- In a diffraction experiment of a native crystal, intensity of reflection (-1 0 6) is equivalent to the intensity of reflection (1 0 -6). true or false?arrow_forwardin an x-ray diffraction experiment, moving the detector farther away from the crystal will allow collection of reflection of reflections with high Miller indices. true or false?arrow_forwardShow the mechanism for the acid-catalyzed formation of an [α-1,6] glycosidic linkagebetween two molecules of α-D-glucopyranose.arrow_forward
- BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage LearningBiology: The Dynamic Science (MindTap Course List)BiologyISBN:9781305389892Author:Peter J. Russell, Paul E. Hertz, Beverly McMillanPublisher:Cengage LearningAnatomy & PhysiologyBiologyISBN:9781938168130Author:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark WomblePublisher:OpenStax College
- BiochemistryBiochemistryISBN:9781305961135Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougalPublisher:Cengage LearningBiology: The Unity and Diversity of Life (MindTap...BiologyISBN:9781305073951Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa StarrPublisher:Cengage LearningHuman Heredity: Principles and Issues (MindTap Co...BiologyISBN:9781305251052Author:Michael CummingsPublisher:Cengage Learning





