BIOCHEMISTRY
BIOCHEMISTRY
8th Edition
ISBN: 9781319296186
Author: BERG
Publisher: MAC HIGHER
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Chapter 3, Problem 4P
Interpretation Introduction

Interpretation:

The absorbance of a 1 mg/mL solution across a 1-cm path should be determined along with the percentage of transmission of incident-light.

Concept introduction:

The absorbance(A) of a solution is represented as

A=log10(I0/I)

In this equation I0 is the incident-light intensity.

Transmitted-light intensity is denoted by I.

The relation between extinction coefficient, concentration and path length is denoted as l (in cm) as:

A = εlc

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