Microbiology: An Introduction (13th Edition)
13th Edition
ISBN: 9780134605180
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
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Question
Chapter 3, Problem 2A
Summary Introduction
To write:
The use of a good compound light microscope with a resolving power of 0.3 mm, a 10x and a 100x oil immersion lens.
Given:
“Compound light microscope” and “resolving power”
Introduction:
A compound microscope is consists of two convex lenses with shorter focal length, used to magnify the tiny objects. The resolving power of the compound microscope is 0.2µm and magnification power is 2000x.
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Using a good compound light microscope with a resolving of 0.3 micro meter a 10x ocular lens ,and a 100 x oil immersion lens, would you be able to discern two objects separated by 0.4 micro meter?0.2 micro meter? 300 neno meter ? Explain your reasoning.
Calculate the diameter of the field of view for each total magnification on your microscope in millimeters
(mm) and then convert this value to micrometers (um):
4.5 mm =
4,500 um
Scanning (40X):
1.8 mm x 100X/40X =
Low power (100X):
FOV diameter 1.8 mm =
1,800 um
High power (400X):
1.8 mm x 100X/400X = 0.45 mm =
450 um
Oil immersion (1000X):
O.18m
180 um
1.8 mm x 100x/1000X =
Draw and estimate the length of a single Euglena (high power) and Paramecium (low power):
Paramecium
Euglena
total magnification
total magnification
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yoox
FOV diam.
FOV diam.
um
uni
length
length
um
In confocal microscopy, what is the theoretical resolving power of the objective used (63X, NA 1.3, oil immersion noil=1.52)? Clearly show your calculations.
Chapter 3 Solutions
Microbiology: An Introduction (13th Edition)
Ch. 3 - Fill in the following blanks. 1. 1 m = ________ m...Ch. 3 - Prob. 2RCh. 3 - Prob. 3RCh. 3 - Prob. 4RCh. 3 - Prob. 5RCh. 3 - Why is a mordant used in the Gram stain? In the...Ch. 3 - Prob. 7RCh. 3 - Prob. 8RCh. 3 - Fill in the following table regarding the Gram...Ch. 3 - NAME IT A sputum sample from Calle, a 30-year-old...
Ch. 3 - Through the microscope, the green structures are...Ch. 3 - Prob. 2MCQCh. 3 - Carbolfuchsin can be used as a simple stain and a...Ch. 3 - Prob. 4MCQCh. 3 - Which of the following is not a functionally...Ch. 3 - Which of the following pairs is mismatched? 1....Ch. 3 - Assume you stain Clostridium by applying a basic...Ch. 3 - Prob. 8MCQCh. 3 - In 1996, scientists described a new tapeworm...Ch. 3 - Prob. 10MCQCh. 3 - Prob. 1ACh. 3 - Prob. 2ACh. 3 - Why isnt the Gram stain used on acid-fast...Ch. 3 - Endospores can be seen as refractile structures in...Ch. 3 - In 1882, German bacteriologist Paul Erhlich...Ch. 3 - Laboratory diagnosis of Neisseria gonorrhoeae...Ch. 3 - Assume that you are viewing a Gram-stained sample...
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