A gram staining is a differential stain most commonly used to classify bacteria in to gram-positive and gram-negative groups. It uses a primary stain (crystal violet), a decolorizer (alcohol/acetone) and a counter stain (safranin).

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Chapter 3, Problem 8MCQ

Summary Introduction

Introduction:

A gram staining is a differential stain most commonly used to classify bacteria in to gram-positive and gram-negative groups. It uses a primary stain (crystal violet), a decolorizer (alcohol/acetone) and a counter stain (safranin).

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Describe in detail all the steps needed to determine whether an Unknown bacteria has a Gram-positive or Gram-negative cell wall using the Gram stain. (You can describe the method presented in the Exercise 3-6 Gram stain videos or in the Lab Manual.) 1. Start by describing the method of transferring the bacteria onto the microscope slide, then describe heat-fixing the emulsion of the Unknown bacteria. (Do not describe all the steps needed to perform aseptic technique. Just say “Bacteria was added aseptically.”) 2. Describe the Gram stain in detail. (This description should include all the stains, how long the stain should set on slide, and Genus and species names of bacteria you are using as CONTROLS.)

Based from the results obtained from Gram staining, explain why bacterial cells retained or changed their color. Briefly explain the principle behind the procedure. Cite advantages of differential staining method (Gram staining and endospore staining) over simple staining only. List other staining methods aside from those you performed in the laboratory. conclusion:

After conducting the Gram staining procedures and you observe nothing (you didn’t see anything) in your slide under the microscope, what could be the mistake you have done? What can you observe when you view a Gram stained-mixture of bacterial specimen?

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Question

Chapter 3, Problem 8MCQ

Summary Introduction

Introduction:

A gram staining is a differential stain most commonly used to classify bacteria in to gram-positive and gram-negative groups. It uses a primary stain (crystal violet), a decolorizer (alcohol/acetone) and a counter stain (safranin).

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Question

Chapter 3, Problem 8MCQ

Summary Introduction

Introduction:

A gram staining is a differential stain most commonly used to classify bacteria in to gram-positive and gram-negative groups. It uses a primary stain (crystal violet), a decolorizer (alcohol/acetone) and a counter stain (safranin).

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Question

Chapter 3, Problem 8MCQ

Summary Introduction

Introduction:

A gram staining is a differential stain most commonly used to classify bacteria in to gram-positive and gram-negative groups. It uses a primary stain (crystal violet), a decolorizer (alcohol/acetone) and a counter stain (safranin).

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Answer 5

Chapter 3, Problem 8MCQ

Summary Introduction

Introduction:

A gram staining is a differential stain most commonly used to classify bacteria in to gram-positive and gram-negative groups. It uses a primary stain (crystal violet), a decolorizer (alcohol/acetone) and a counter stain (safranin).

Answer 6

Chapter 3, Problem 8MCQ

Summary Introduction

Introduction:

A gram staining is a differential stain most commonly used to classify bacteria in to gram-positive and gram-negative groups. It uses a primary stain (crystal violet), a decolorizer (alcohol/acetone) and a counter stain (safranin).

Answer 7

Chapter 3, Problem 8MCQ

Summary Introduction

Introduction:

A gram staining is a differential stain most commonly used to classify bacteria in to gram-positive and gram-negative groups. It uses a primary stain (crystal violet), a decolorizer (alcohol/acetone) and a counter stain (safranin).

Answer 8

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Ch. 3 - Fill in the following blanks. 1. 1 m = ________ m...Ch. 3 - Prob. 2R Ch. 3 - Prob. 3R Ch. 3 - Prob. 4R Ch. 3 - Prob. 5R Ch. 3 - Why is a mordant used in the Gram stain? In the...Ch. 3 - Prob. 7R Ch. 3 - Prob. 8R Ch. 3 - Fill in the following table regarding the Gram...Ch. 3 - NAME IT A sputum sample from Calle, a 30-year-old...

A gram staining is a differential stain most commonly used to classify bacteria in to gram-positive and gram-negative groups. It uses a primary stain (crystal violet), a decolorizer (alcohol/acetone) and a counter stain (safranin).

Solution Summary: The author explains that a gram staining is based on the presence or absence of peptidoglycan in bacterial cells.

Concept explainers

Introduction:

A gram staining is a differential stain most commonly used to classify bacteria in to gram-positive and gram-negative groups. It uses a primary stain (crystal violet), a decolorizer (alcohol/acetone) and a counter stain (safranin).

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Chapter 3 Solutions

A gram staining is a differential stain most commonly used to classify bacteria in to gram-positive and gram-negative groups. It uses a primary stain (crystal violet), a decolorizer (alcohol/acetone) and a counter stain (safranin).

Solution Summary: The author explains that a gram staining is based on the presence or absence of peptidoglycan in bacterial cells.

Concept explainers

Introduction: A gram staining is a differential stain most commonly used to classify bacteria in to gram-positive and gram-negative groups. It uses a primary stain (crystal violet), a decolorizer (alcohol/acetone) and a counter stain (safranin).

Want to see the full answer?

Chapter 3 Solutions

Introduction:

A gram staining is a differential stain most commonly used to classify bacteria in to gram-positive and gram-negative groups. It uses a primary stain (crystal violet), a decolorizer (alcohol/acetone) and a counter stain (safranin).