Concept explainers
(a)
To explain: How Sanger’s results support the results of the given experiment.
Introduction:
Proteins are the
(b)
To check: Whether the results for the known structure of bovine insulin is consistent or not.
Introduction:
Proteins are composed of various amino acids residues through polymerization. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.
(c)
To explain: The first four amino acids of the B chain based on the given data.
Introduction:
Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.
Given:
B1: α-DNP-phenylalanine only
B2: α-DNP-phenylalanine; valine
B3: aspartic acid; α-DNP-phenylalanine; valine
B4: aspartic acid; glutamic acid; α-DNP-phenylalanine; valine
(d1)
To explain: Whether results match the known sequence of bovine insulin.
Introduction:
Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.
(d2)
To determine: If there are any discrepancies in the results when matched with the known sequence of bovine insulin.
Introduction:
Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.
(e)
To explain: How the amino acid sequence of A chain was determined.
Introduction:
Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.
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Lehninger Principles of Biochemistry
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