Concept explainers
(a)
To explain: How Sanger’s results support the results of the given experiment.
Introduction:
Proteins are the
(b)
To check: Whether the results for the known structure of bovine insulin is consistent or not.
Introduction:
Proteins are composed of various amino acids residues through polymerization. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.
(c)
To explain: The first four amino acids of the B chain based on the given data.
Introduction:
Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.
Given:
B1: α-DNP-phenylalanine only
B2: α-DNP-phenylalanine; valine
B3: aspartic acid; α-DNP-phenylalanine; valine
B4: aspartic acid; glutamic acid; α-DNP-phenylalanine; valine
(d1)
To explain: Whether results match the known sequence of bovine insulin.
Introduction:
Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.
(d2)
To determine: If there are any discrepancies in the results when matched with the known sequence of bovine insulin.
Introduction:
Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.
(e)
To explain: How the amino acid sequence of A chain was determined.
Introduction:
Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

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Chapter 3 Solutions
SAPLINGPLUS FOR PRINCIPLES OF BIOCHEMIS
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- Problem 6-37 Draw the different monochlorinated constitutional isomers you would obtain by the radical chlorination of the following compounds. (b) (c) Problem 6-39 Show the structure of the carbocation that would result when each of the following alkenes reacts with an acid, H+. (a) (b) (c)arrow_forwardPlease draw the major product of this reaction. Ignore inorganic byproducts and the carboxylic side productarrow_forwardPlease draw the major product of this reaction.arrow_forward
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