SaplingPlus for Lehninger Principles of Biochemistry (Six-Month Access)
SaplingPlus for Lehninger Principles of Biochemistry (Six-Month Access)
7th Edition
ISBN: 9781319108236
Author: David L. Nelson, Michael M. Cox
Publisher: W. H. Freeman
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Chapter 3, Problem 23DAP

(a)

Summary Introduction

To explain: How Sanger’s results support the results of the given experiment.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(b)

Summary Introduction

To check: Whether the results for the known structure of bovine insulin is consistent or not.

Introduction:

Proteins are composed of various amino acids residues through polymerization. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(c)

Summary Introduction

To explain: The first four amino acids of the B chain based on the given data.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Given:

B1: α-DNP-phenylalanine only

B2: α-DNP-phenylalanine; valine

B3: aspartic acid; α-DNP-phenylalanine; valine

B4: aspartic acid; glutamic acid; α-DNP-phenylalanine; valine

(d1)

Summary Introduction

To explain: Whether results match the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(d2)

Summary Introduction

To determine: If there are any discrepancies in the results when matched with the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(e)

Summary Introduction

To explain: How the amino acid sequence of A chain was determined.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

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