Chapter 3, Problem 15P

(a)

Summary Introduction

To calculate: Specific activity of the enzyme after each purification procedure.

Introduction:

Specific activity determines the activity of a particular enzyme per milligram of all the protein. Specific activity of a particular enzyme can be calculated as follows:

$\text{Specific}\text{activity}=\frac{\text{Units}\text{of}\text{enzyme}\text{activity}}{\text{milligrams}\text{of}\text{protein}}$

(b)

Summary Introduction

To determine: The most effective purification procedure used for the given enzyme.

Introduction:

In ion-exchange chromatography, polar molecules or ions are separated on the basis of their affinity to ion exchanger. Proteins, amino acids, and small nucleotides can be seprated with the help of ion exchange chromatography.

(c)

Summary Introduction

To determine: The least effective purification procedure used for the enzyme.

Introduction:

$\text{pH}$ precipitation is also termed as protein precipitation. This process is widely being used in downstream processing to concentrate and purify proteins.

(d)

Summary Introduction

To determine:  Whether the enzyme is pure after step 6 and what could be done to estimate the purity of the enzyme preparation. Also, to determine any other procedure that could estimate the purity of enzyme preparation.

Introduction:

Enzyme purification is a process to purify and concentrate the enzymes. It gives knowledge about their functional and structural properties and also foretells their applications. There are various methods that can be used for enzyme purification. These include centrifugation, filtration, ultrafiltration, precipitation, chromatography, and many others.