Chapter 3, Problem 18E

Summary Introduction

To describe: The selection procedure of recombinant clones if a foreign DNA is introduced into the polylinker site of pUC18 and introduced into E.coli cells.

Concept introduction:  Cloning is defined as the production of multiple identical organisms obtained from a single ancestor. The clone is the collection of cells that carry the vector carrying DNA of interest. To clone a particular DNA fragment, a pure form of DNA is obtained and cut using a restriction enzyme into small pieces. Then these fragments are cloned into a vector.