FUNDAMENTALS OF BIOCHEMISTRY WPNG 1-SEME
5th Edition
ISBN: 9781119750192
Author: Voet
Publisher: WILEY
expand_more
expand_more
format_list_bulleted
Question
Chapter 3, Problem 19E
Summary Introduction
To determine: The restriction enzymes would interfere with the production of β-galactosidase by the host cell.
Concept introduction: The plasmid pUC18 is a circular plasmid that contains multiple restriction sites. In pUC18, the enzyme β-galactosidase is encoded by the lacZ gene. The β-galactosidase cleaves the colorless compound X-gal to a blue-colored product.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
please draw it for me and tell me where i need to modify the structure
Please help determine the standard curve for my Kinase Activity in Excel Spreadsheet.
Link: https://mnscu-my.sharepoint.com/personal/vi2163ss_go_minnstate_edu/_layouts/15/Doc.aspx?sourcedoc=%7B958f5aee-aabd-45d7-9f7e-380002892ee0%7D&action=default&slrid=9b178ea1-b025-8000-6e3f-1cbfb0aaef90&originalPath=aHR0cHM6Ly9tbnNjdS1teS5zaGFyZXBvaW50LmNvbS86eDovZy9wZXJzb25hbC92aTIxNjNzc19nb19taW5uc3RhdGVfZWR1L0VlNWFqNVc5cXRkRm4zNDRBQUtKTHVBQldtcEtWSUdNVmtJMkoxQzl3dmtPVlE_cnRpbWU9eEE2X291ZHIzVWc&CID=e2126631-9922-4cc5-b5d3-54c7007a756f&_SRM=0:G:93
Determine the amount of VRK1 is present
1. Average the data and calculate the mean absorbance for each concentration/dilution (Please over look for Corrections)
2. Blank Correction à Subtract 0 ug/mL blank absorbance from all readings (Please over look for Corrections)
3. Plot the Standard Curve (Please over look for Corrections)
4. Convert VRK1 concentration from ug/mL to g/L
5. Use the molar mass of VRK1 to convert to M and uM…
Macmillan Learning
Cholesterol synthesis begins with the formation of mevalonate from acetyl CoA. This
process activates mevalonate and converts it to isopentenyl pyrophosphate.
Identify the atoms in mevalonate and isopentenyl pyrophosphate that will be labeled
from acetyl CoA labeled with 14C in the carbonyl carbon. Place 14C atoms and C atoms
to denote which carbon atoms are labeled and which are not labeled.
H₂C
COA
14C-labeled acetyl-CoA
HHH
[c]
H
H
OH
014C
- OH
H
HH
H
Mevalonate
CH3
H H
14C
H
Η
H
H
Incorrect Answer
of o
-P-O-P-0-
Isopentenyl pyrophosphate
с
Answer Bank
14C
Chapter 3 Solutions
FUNDAMENTALS OF BIOCHEMISTRY WPNG 1-SEME
Ch. 3 - Prob. 1ECh. 3 - 2. Name the following nucleotide.
Ch. 3 - 3. In many organisms, DNA is modified by...Ch. 3 - 4. When cytosine is treated with bisulfite, the...Ch. 3 - 5. Kinases are enzymes that transfer a phosphoryl...Ch. 3 - 6. Kinases are enzymes that transfer a phosphoryl...Ch. 3 - 7. A diploid organism with a 45,000-kb haploid...Ch. 3 - 8. A segment of DNA containing 20 base pairs...Ch. 3 - 9. Explain why the strands of a DNA molecule can...Ch. 3 - 10. Explain why increasing the NaCl concentration...
Ch. 3 - 11. An enzyme from the human immunodeficiency...Ch. 3 - 12. How many different amino acids could...Ch. 3 - 13. The human genome contains thousands of...Ch. 3 - 14. By how many nucleotides, on average, do the...Ch. 3 - 15. The recognition sequence for the restriction...Ch. 3 - 16. Using the data in Table 3-2, identify...Ch. 3 - 17. The 13-Mb genome of the green alga...Ch. 3 - 18. Describe how to select recombinant clones if a...Ch. 3 - Prob. 19ECh. 3 - Prob. 20ECh. 3 - 21. Why is a genomic library larger than a cDNA...Ch. 3 - 22. Why do cDNA libraries derived from different...Ch. 3 - 23. Some RNA molecules me covalently modified by...Ch. 3 - 24. Would the modified nucleoside described in...Ch. 3 - 25. Draw the tautomeric form of adenine.
Ch. 3 - 26. Draw the tautomeric form of cytosine.
Ch. 3 - 27. The pK value for N1 of adenine is 3.64,...Ch. 3 - Prob. 28CQCh. 3 - Prob. 29CQCh. 3 - 30. Hypoxanthine can also base-pair with cytosine....Ch. 3 - 31. Describe the outcome of a chain-terminator...Ch. 3 - 32. Describe the outcome of a chain-terminator...Ch. 3 - 33. Calculate the number of clones required to...Ch. 3 - 34. You are attempting to clone a 250-kb segment...Ch. 3 - 35. Describe the possible outcome of a PCR...Ch. 3 - 36. Describe the possible outcome of a PCR...Ch. 3 - 37. Write the sequences of the two 12-residue...Ch. 3 - 38. A blood slain from a crime scene and blood...Ch. 3 - Prob. 1MTE
Knowledge Booster
Similar questions
- Draw the reaction between sphingosine and arachidonic acid. Draw out the full structures.arrow_forwardDraw both cis and trans oleic acid. Explain why cis-oleic acid has a melting point of 13.4°C and trans-oleic acid has a melting point of 44.5°C.arrow_forwardDraw the full structure of the mixed triacylglycerol formed by the reaction of glycerol and the fatty acids arachidic, lauric and trans-palmitoleic. Draw the line structure.arrow_forward
- Draw out the structure for lycopene and label each isoprene unit. "Where is lycopene found in nature and what health benefits does it provide?arrow_forwardWhat does it mean to be an essential fatty acid? What are the essential fatty acids?arrow_forwardCompare and contrast primary and secondary active transport mechanisms in terms of energy utilisation and efficiency. Provide examples of each and discuss their physiological significance in maintaining ionic balance and nutrient uptake. Rubric Understanding the key concepts (clearly and accurately explains primary and secondary active transport mechanisms, showing a deep understanding of their roles) Energy utilisation analysis ( thoroughly compares energy utilisation in primary and secondary transport with specific and relevant examples Efficiency discussion Use of examples (provides relevant and accurate examples (e.g sodium potassium pump, SGLT1) with clear links to physiological significance. Clarity and structure (presents ideas logically and cohesively with clear organisation and smooth transition between sections)arrow_forward
- 9. Which one of the compounds below is the major organic product obtained from the following reaction sequence, starting with ethyl acetoacetate? 요요. 1. NaOCH2CH3 CH3CH2OH 1. NaOH, H₂O 2. H3O+ 3. A OCH2CH3 2. ethyl acetoacetate ii A 3. H3O+ OH B C D Earrow_forward7. Only one of the following ketones cannot be made via an acetoacetic ester synthesis. Which one is it? Ph کہ A B C D Earrow_forward2. Which one is the major organic product obtained from the following reaction sequence? HO A OH 1. NaOEt, EtOH 1. LiAlH4 EtO OEt 2. H3O+ 2. H3O+ OH B OH OH C -OH HO -OH OH D E .CO₂Etarrow_forward
- what is a protein that contains a b-sheet and how does the secondary structure contributes to the overall function of the protein.arrow_forwarddraw and annotate a b-sheet and lable the hydrogen bonding. what is an example that contains the b-sheet and how the secondary structure contributes to the overall function of your example protein.arrow_forwardFour distinct classes of interactions (inter and intramolecular forces) contribute to a protein's tertiary and quaternary structures. Name the interaction then describe the amino acids that can form this type of interaction. Draw and annotate a diagram of the interaction between two amino acids.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
BiochemistryBiochemistryISBN:9781319114671Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.Publisher:W. H. Freeman
Lehninger Principles of BiochemistryBiochemistryISBN:9781464126116Author:David L. Nelson, Michael M. CoxPublisher:W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul...BiochemistryISBN:9781118918401Author:Donald Voet, Judith G. Voet, Charlotte W. PrattPublisher:WILEY
BiochemistryBiochemistryISBN:9781305961135Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougalPublisher:Cengage Learning
BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning
Fundamentals of General, Organic, and Biological ...BiochemistryISBN:9780134015187Author:John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. PetersonPublisher:PEARSON
Biochemistry
Biochemistry
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:9781464126116
Author:David L. Nelson, Michael M. Cox
Publisher:W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul...
Biochemistry
ISBN:9781118918401
Author:Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:WILEY
Biochemistry
Biochemistry
ISBN:9781305961135
Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:Cengage Learning
Biochemistry
Biochemistry
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Cengage Learning
Fundamentals of General, Organic, and Biological ...
Biochemistry
ISBN:9780134015187
Author:John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:PEARSON