The reason for the difference in the molecular weight calculated from SDS-PAGE gel and from the molecular weight should be suggested. Concept introduction: A peptide bond is present between the two amino acids in a protein. During the formation of a peptide bond, a molecule of water is released. The amino group of an amino acid gets associated with the carboxyl group of another. Polypeptides and proteins are the chains formed by the amino acids.

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Please help determine the standard curve for my Kinase Activity in Excel Spreadsheet. Link: https://mnscu-my.sharepoint.com/personal/vi2163ss_go_minnstate_edu/_layouts/15/Doc.aspx?sourcedoc=%7B958f5aee-aabd-45d7-9f7e-380002892ee0%7D&action=default&slrid=9b178ea1-b025-8000-6e3f-1cbfb0aaef90&originalPath=aHR0cHM6Ly9tbnNjdS1teS5zaGFyZXBvaW50LmNvbS86eDovZy9wZXJzb25hbC92aTIxNjNzc19nb19taW5uc3RhdGVfZWR1L0VlNWFqNVc5cXRkRm4zNDRBQUtKTHVBQldtcEtWSUdNVmtJMkoxQzl3dmtPVlE_cnRpbWU9eEE2X291ZHIzVWc&CID=e2126631-9922-4cc5-b5d3-54c7007a756f&_SRM=0:G:93 Determine the amount of VRK1 is present 1. Average the data and calculate the mean absorbance for each concentration/dilution (Please over look for Corrections) 2. Blank Correction à Subtract 0 ug/mL blank absorbance from all readings (Please over look for Corrections) 3. Plot the Standard Curve (Please over look for Corrections) 4. Convert VRK1 concentration from ug/mL to g/L 5. Use the molar mass of VRK1 to convert to M and uM…

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Question

Chapter 3, Problem 10P

Interpretation Introduction

Interpretation:

The reason for the difference in the molecular weight calculated from SDS-PAGE gel and from the molecular weight should be suggested.

Concept introduction:

A peptide bond is present between the two amino acids in a protein. During the formation of a peptide bond, a molecule of water is released. The amino group of an amino acid gets associated with the carboxyl group of another. Polypeptides and proteins are the chains formed by the amino acids.

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