The importance of virus in heat tolerance due to the symbiosis between Dichanthelium lanuginosu and Curvularia protuberata as demonstrated by Márquez and associates. Introduction: When two organisms interact with each other in order to survive, it is known as a symbiotic relationship and both the organisms living in a symbiotic relationship gain equal benefits. Mutualism, competition, parasitism , and commensalism are the four main types of symbiotic relationships. When two species choose to live together, it is known as facultative symbiosis and when two species live together in order to survive, it is known as obligate symbiosis.

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Answer 1

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Definition Definition Organism that lives in the body of another organism and obtains food at the expense of the host. A parasite can directly or indirect harm the host. There are two kinds of parasites: internal and external. An internal parasite lives inside another organism and is known as an endoparasite. An external parasite lives on the body surface of the host and is known as an ectoparasite. Protozoa and helminths are examples of major human parasites.

Chapter 29.4, Problem 2EQ

Summary Introduction

To determine: The importance of virus in heat tolerance due to the symbiosis between Dichanthelium lanuginosu and Curvularia protuberata as demonstrated by Márquez and associates.

Introduction: When two organisms interact with each other in order to survive, it is known as a symbiotic relationship and both the organisms living in a symbiotic relationship gain equal benefits. Mutualism, competition, parasitism, and commensalism are the four main types of symbiotic relationships. When two species choose to live together, it is known as facultative symbiosis and when two species live together in order to survive, it is known as obligate symbiosis.

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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results? 2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab? 3) Why are the cells incubated at 42°C?

Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…

Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…

Answer 2

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Definition Definition Organism that lives in the body of another organism and obtains food at the expense of the host. A parasite can directly or indirect harm the host. There are two kinds of parasites: internal and external. An internal parasite lives inside another organism and is known as an endoparasite. An external parasite lives on the body surface of the host and is known as an ectoparasite. Protozoa and helminths are examples of major human parasites.

Chapter 29.4, Problem 2EQ

Summary Introduction

To determine: The importance of virus in heat tolerance due to the symbiosis between Dichanthelium lanuginosu and Curvularia protuberata as demonstrated by Márquez and associates.

Introduction: When two organisms interact with each other in order to survive, it is known as a symbiotic relationship and both the organisms living in a symbiotic relationship gain equal benefits. Mutualism, competition, parasitism, and commensalism are the four main types of symbiotic relationships. When two species choose to live together, it is known as facultative symbiosis and when two species live together in order to survive, it is known as obligate symbiosis.

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Answer 3

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Definition Definition Organism that lives in the body of another organism and obtains food at the expense of the host. A parasite can directly or indirect harm the host. There are two kinds of parasites: internal and external. An internal parasite lives inside another organism and is known as an endoparasite. An external parasite lives on the body surface of the host and is known as an ectoparasite. Protozoa and helminths are examples of major human parasites.

Chapter 29.4, Problem 2EQ

Summary Introduction

To determine: The importance of virus in heat tolerance due to the symbiosis between Dichanthelium lanuginosu and Curvularia protuberata as demonstrated by Márquez and associates.

Introduction: When two organisms interact with each other in order to survive, it is known as a symbiotic relationship and both the organisms living in a symbiotic relationship gain equal benefits. Mutualism, competition, parasitism, and commensalism are the four main types of symbiotic relationships. When two species choose to live together, it is known as facultative symbiosis and when two species live together in order to survive, it is known as obligate symbiosis.

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Answer 4

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Definition Definition Organism that lives in the body of another organism and obtains food at the expense of the host. A parasite can directly or indirect harm the host. There are two kinds of parasites: internal and external. An internal parasite lives inside another organism and is known as an endoparasite. An external parasite lives on the body surface of the host and is known as an ectoparasite. Protozoa and helminths are examples of major human parasites.

Chapter 29.4, Problem 2EQ

Summary Introduction