Microbiology: An Introduction
Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Chapter 28, Problem 3CAE

The antibiotic efrotomycin is produced by Streptomyces lactamdurans. S. lactamdurans was grown in 40,000 liters of medium. The medium consisted of glucose, maltose, soybean oil, (NH4)2SO4, NaCl, KH2PO4, and Na2HPO4. The culture was aerated and maintained at 28°C. The following results were obtained from analyses of the culture medium during cell growth:

Chapter 28, Problem 3CAE, The antibiotic efrotomycin is produced by Streptomyces lactamdurans. S. lactamdurans was grown in , example  1

Chapter 28, Problem 3CAE, The antibiotic efrotomycin is produced by Streptomyces lactamdurans. S. lactamdurans was grown in , example  2

  1. a. Under what conditions is the most efrotomycin produced? Is it a primary or secondary metabolite?
  2. b. Which is used first, maltose or glucose? Suggest a reason for this.
  3. c. What is the purpose of each ingredient in the growth medium? (Hint: See Chapter 6.)
  4. d. What is Streptomyces? (Hint: See Chapter 11.)
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Microbiology: An Introduction

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