Two components in an HPLC separation have retention times that differ by 15 s. The first peak elutes in 9.0 min, and the peak widths are approximately equal. The dead time to was 65 s. Use a spreadsheet to find the minimum number of theoretical plates needed to achieve the following resolution. R. values: 0.50 0.75, 0.90, 1.0, 1.10. 1.25, 1.50. L75. 2.0 and 2.5. How would the results change if peak 2 were twice as broad as peak 1

Q: The chromatogram in the figure is of a mixture of 2 compounds, carried out in a packed liquid…

A: Length of column (L) = 25 cmTime of non-retained component (t) = 5 minFrom the given curve, with the…

Q: In lon Chromatography retention times and the resolution between adjacent peaks can be affected by…

A: This concept is based on the properties of solutions, where the solute (in this case, ions) is…

Q: The following data were obtained by gas chromatography for a 30-cm packed column: Compound Retention…

A: selectivity factor is 0.22 Explanation:From the given data, we can calculate the selectivity factor…

Q: Explain the relationship between polarity of the compound and retention factor in chromatography

A: In chromatography, retention factor is the ratio of the distance travelled by the individual…

Q: A chromatogram gives an ideal Gaussian peak with tR = 9.40 min and win = 0.19 min. How many…

A: Chromatography is a techniques where separation of components takes places depending on various…

Q: The solid material in column chromatography is typically referred to as: 1) the stationary phase 2)…

A: Chromatography is a technique which is used for the separation and purification of components in…

Q: A mixture of A and B is injected into a liquid chromatographic column with length of 24.8 cm. The…

A: Given :  Separation resolution between A and B can be calculated using formula: Rs = 2RtB-RtAw1 +…

Q: A solute with a retention time of 407 seconds has a width at the base of 13.0 seconds on a column…

A: The objective of the question is to calculate the Number of Theoretical PlatesPlate Height

Q: A mixture of three components is separated using paper chromatography, and each component is…

A: Given: Absorbance of standard solution- Compound A = 0.72 Compound B = 0.55 Compound C = 0.80 For…

Q: A mixture of A and B is injected into a liquid chromatographic column with length of 45 cm. The…

A: given- length of the plate= L = 45 cm plate height = H = ?

Q: Consider a separation performed on a 45.0 mm long open tubular column with a 0.50 mm diameter and a…

A:

Q: The following is an HPLC separation of deuterated benzenes on a C18 column that 440 cm long. The…

A: 1. mobile phase velocity (<v>) = 10.552 cm/min 2. retention factor (k') of C6D6 is 3.511 3.…

Q: A student used thin layer chromatography to confirm whether he could successfully separate two…

A: TLC means “Thin Layer Chromatography”. This experiment is done on Wattman paper. If the molecule has…

Mass Spectrometry

This process is an instrumental method in which a sample is changed to fast moving positive ions by electron bombardment. The charged particles vary due to their different mass to charge ratio and are estimated with the help of this difference. It is also called mass spectroscopy.